TY - JOUR
T1 - Antigen-specific murine T-cell lymphomas
T2 - Functional heterogenicity
AU - Azar, Yehudith
AU - Eidelsztein, Paula
AU - Yefenof, Eitan
AU - Chriqui, Evelyne
AU - Katz-Gross, Aviva
AU - Kedar, Eli
AU - Ben-Sasson, Shlomo Zalman
PY - 1981/11/15
Y1 - 1981/11/15
N2 - Two ovalbumin (OVA)-specific helper lymphomas (designated ROT/6.1 and ROT/6.2) were established by transformation of enriched, OVA-immune T cells with the radiation leukemia virus (RadLV). Shortly after establishment these lymphomas provided carrier (OVA)-specific help for anti-hapten antibody response. However, 5 months later ROT/6.1 lost its OVA specificity and could augment anti-hapten antibody response in the presence of an unrelated carrier. ROT/6.2 retained its antigen-specific helper function over 10 months of repeated passaging. This OVA-specific helper line inhibited anti-hapten antibody response when given together with an unrelated carrier. Cloning of ROT/6.2 by limiting dilution revealed that only 3 of 10 clones tested had OVA-specific helper activity. None of the clones could induce antigen-specific DTH reaction. The interrelationship between the functional heterogenicity, specificity, and stability of the helper lines is discussed.
AB - Two ovalbumin (OVA)-specific helper lymphomas (designated ROT/6.1 and ROT/6.2) were established by transformation of enriched, OVA-immune T cells with the radiation leukemia virus (RadLV). Shortly after establishment these lymphomas provided carrier (OVA)-specific help for anti-hapten antibody response. However, 5 months later ROT/6.1 lost its OVA specificity and could augment anti-hapten antibody response in the presence of an unrelated carrier. ROT/6.2 retained its antigen-specific helper function over 10 months of repeated passaging. This OVA-specific helper line inhibited anti-hapten antibody response when given together with an unrelated carrier. Cloning of ROT/6.2 by limiting dilution revealed that only 3 of 10 clones tested had OVA-specific helper activity. None of the clones could induce antigen-specific DTH reaction. The interrelationship between the functional heterogenicity, specificity, and stability of the helper lines is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0019816289&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(81)90064-2
DO - 10.1016/0008-8749(81)90064-2
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C2 - 6172200
AN - SCOPUS:0019816289
SN - 0008-8749
VL - 65
SP - 194
EP - 200
JO - Cellular Immunology
JF - Cellular Immunology
IS - 1
ER -
