Abstract
An investigation was made into the antigenic specificity of the cellular immune response to low molecular weight compounds containing benzenearsonic acid and into the immunogenicity of these compounds. The most significant finding was that the free benzenoid hapten, p-aminobenzenearsonic acid (arsanilic acid), underivatized and with no side chain, triggered a cellular immune response in guinea pigs. The specificity of this response was entirely directed at the aromatic ring and its arsonate substituent, as indicated by the failure of cells from animals primed with this antigen to distinguish between the immunogen and its diazo derivatives with acetyltyrosine, acetylhistidine or ε-aminocaproic acid. The analogue p-nitrobenzenearsonic acid was not immunogenic. Benzenearsonic acid was diazo-bonded to free amino acid and synthetic copolymer carriers. The specificity of the immune response to these types of conjugates included contributions from the side chains. T-lymphocytes from guinea pigs immunized against ABA-acetyltyrosine or ABA-acetylhistidine responded preferentially to the immunogen. This result confirmed the aromatic side chain contribution to specificity which has been demonstrated by other authors. In further concord with the role of a proximal aromatic amino acid in the structure of an ABA-containing hapten was the response pattern of cells from animals immunized with ABA on the copolymer GT. These responded to the immunogen and to ABA-Actyr as well as to various ABA-proteins, but not to ABA-GL. Cells from ABA-GL-primed animals responded to ABA-proteins, but their responsiveness to ABA on the copolymers varied according to their capacity to respond to GL. Responders to GL did not respond to ABA-GT, while a GL non-responder recognized ABA-GT but not ABA-GL.
Original language | English |
---|---|
Pages (from-to) | 1011-1018 |
Number of pages | 8 |
Journal | Molecular Immunology |
Volume | 17 |
Issue number | 8 |
DOIs | |
State | Published - 1980 |
Externally published | Yes |
Keywords
- 2,4-dinitrophenyl
- ABA
- Achis
- Actyr
- aminocaproic acid
- azobenzenearsonate
- cap
- CFA
- complete Freund's adjuvant
- DNP
- GL
- GPA
- GT
- guinea pig serum albumin
- hen ovalbumin
- keyhole limpet hemocyanin
- KLH
- N-acetylhistidine
- N-acetyltyrosine
- OA
- PELs
- peritoneal exudate lymphocytes
- poly-l-glutamic acid: 1-tyrosine 1:1
- poly-l-glutamic acid: l-lysine 6:4
- PPD
- purified protein derivative
- tyr
- tyrosine