Antioxidant and binding properties of methanol extracts from indigo plant leaves

Yun Jum Park, Chang Sik Shin, Bo Eun Kim, Gil Yong Cheon, Jong Hyang Bae, Yang Gyu Ku, Su Min Park, Buk Gu Heo*, Dae Guk Kim, Ja Yong Cho, Shela Gorinstein

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

This study was conducted to clarify the effect of ultra-pressure treatment on the extraction of bioactive compounds from indigo plant leaves (Polygonum tinctorium Lour.) and on their properties. Leaves were harvested the in mid-August, 2013, from Naju City (Korea), and treated using two methods: ultra-pressure (550 MPa, 3 min) and hot-air (70°C, 24 h). Then, the leaves were ultrasonically extracted with methanol. The content of indirubin in leaves treated with ultra pressure and hot air was (535.55 ± 26.14) mg kg -1 and (52.63 ± 6.45) mg kg-1, respectively, and that of tryptanthrin was (165.55 ± 8.74) mg kg-1 and (153.00 ± 7.62) mg kg-1, respectively. Polyphenolic content in the leaves extract was (127.24 ± 13.67) mg kg-1 after the ultrapressure and (88.22 ± 5.33) mg kg-1 after the hot-air treatment. The content of flavonoids was (2298.67 ± 83.27) mg kg -1 after the ultra-pressure and (3224.00 ± 21.45) mg kg -1 after the hotair treatment. Di(phenyl)-(2,4,6-trinitrophenyl) iminoazanium (DPPH) radical scavenging activities of the indigo extract obtained by ultra-pressure and hot-air treatment methods at the concentration of 1 mg mL-1 were (80.25 ± 0.73) % and (66.54 ± 2.35) %, respectively, and 2,2′-azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activities were estimated as (90.14 ± 0.79) % and (64.45 ± 8.97) %, respectively. The methanol leaf extract after ultra-pressure treatment exhibited higher binding properties to human serum albumin in comparison with catechin and conventional treatments. Consequently, it was assumed that the ultra-pressure treatment is an effective method for the extraction of bioactive compounds from indigo leaves.

Original languageEnglish
Pages (from-to)1421-1427
Number of pages7
JournalChemical Papers
Volume68
Issue number10
DOIs
StatePublished - Oct 2014

Keywords

  • antioxidant activity
  • binding properties
  • bioactive compounds
  • indigo leaves

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