Antioxidants Prevention of Diabetic Damage in the Organ Culture Bovine Lenses

Elvira Bormusov; A.    Dovrat; Mordechai Chevion; Abraham Z. Reznick

Antioxidants Prevention of Diabetic Damage in the Organ Culture Bovine Lenses

Elvira Bormusov, A. Dovrat, Mordechai Chevion, Abraham Z. Reznick

Institute of Biomedical Research at the Faculty of Dental Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Oxidative stress represents a mechanism which could lead to diabetic cataract. We exposed bovine lenses inculture conditions for two weeks to high glucose concentration (450 mg%) and investigated the damage to the lens and possible protection by special antioxidants - N-acetyl-L-cysteine (NAC) and the zinc complex of desferrioxamine (DFO), a selective chelator for iron. We monitored the optical quality of the lenses and the oxidation of the epithelium with dichlorofluorescein (DCF) assay, as well as the changes in lens proteins profile by 2D gel electrophoresis. Under high glucose changes in lens focal length, increased oxidation, and changes in lens crystalline were observed. NAC and
Zn- DFO nearly completely protected the lenses; DFO showed only partial protection. The results demonstrated that antioxidants should be considered as treatment modality protecting the lens from high glucose damage. It is proposed
that a combination of NAC and Zn/DFO could prove highly efficient.

Original language	American English
Pages (from-to)	1-7
Journal	Metabolomics : Open Access
Volume	S1
Issue number	4
State	Published - 2012

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title = "Antioxidants Prevention of Diabetic Damage in the Organ Culture Bovine Lenses",

abstract = "Oxidative stress represents a mechanism which could lead to diabetic cataract. We exposed bovine lenses inculture conditions for two weeks to high glucose concentration (450 mg%) and investigated the damage to the lens and possible protection by special antioxidants - N-acetyl-L-cysteine (NAC) and the zinc complex of desferrioxamine (DFO), a selective chelator for iron. We monitored the optical quality of the lenses and the oxidation of the epithelium with dichlorofluorescein (DCF) assay, as well as the changes in lens proteins profile by 2D gel electrophoresis. Under high glucose changes in lens focal length, increased oxidation, and changes in lens crystalline were observed. NAC andZn- DFO nearly completely protected the lenses; DFO showed only partial protection. The results demonstrated that antioxidants should be considered as treatment modality protecting the lens from high glucose damage. It is proposedthat a combination of NAC and Zn/DFO could prove highly efficient.",

author = "Elvira Bormusov and A. Dovrat and Mordechai Chevion and Reznick, {Abraham Z.}",

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language = "American English",

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journal = "Metabolomics : Open Access",

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T1 - Antioxidants Prevention of Diabetic Damage in the Organ Culture Bovine Lenses

AU - Bormusov, Elvira

AU - Dovrat, A.

AU - Chevion, Mordechai

AU - Reznick, Abraham Z.

PY - 2012

Y1 - 2012

N2 - Oxidative stress represents a mechanism which could lead to diabetic cataract. We exposed bovine lenses inculture conditions for two weeks to high glucose concentration (450 mg%) and investigated the damage to the lens and possible protection by special antioxidants - N-acetyl-L-cysteine (NAC) and the zinc complex of desferrioxamine (DFO), a selective chelator for iron. We monitored the optical quality of the lenses and the oxidation of the epithelium with dichlorofluorescein (DCF) assay, as well as the changes in lens proteins profile by 2D gel electrophoresis. Under high glucose changes in lens focal length, increased oxidation, and changes in lens crystalline were observed. NAC andZn- DFO nearly completely protected the lenses; DFO showed only partial protection. The results demonstrated that antioxidants should be considered as treatment modality protecting the lens from high glucose damage. It is proposedthat a combination of NAC and Zn/DFO could prove highly efficient.

AB - Oxidative stress represents a mechanism which could lead to diabetic cataract. We exposed bovine lenses inculture conditions for two weeks to high glucose concentration (450 mg%) and investigated the damage to the lens and possible protection by special antioxidants - N-acetyl-L-cysteine (NAC) and the zinc complex of desferrioxamine (DFO), a selective chelator for iron. We monitored the optical quality of the lenses and the oxidation of the epithelium with dichlorofluorescein (DCF) assay, as well as the changes in lens proteins profile by 2D gel electrophoresis. Under high glucose changes in lens focal length, increased oxidation, and changes in lens crystalline were observed. NAC andZn- DFO nearly completely protected the lenses; DFO showed only partial protection. The results demonstrated that antioxidants should be considered as treatment modality protecting the lens from high glucose damage. It is proposedthat a combination of NAC and Zn/DFO could prove highly efficient.

M3 - Article

SN - 2153-0769

VL - S1

SP - 1

EP - 7

JO - Metabolomics : Open Access

JF - Metabolomics : Open Access

IS - 4

ER -

Antioxidants Prevention of Diabetic Damage in the Organ Culture Bovine Lenses

Abstract

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