APOBEC3B-Mediated Cytidine Deamination Is Required for Estrogen Receptor Action in Breast Cancer

Manikandan Periyasamy; Hetal Patel; Chun Fui Lai; Van T.M. Nguyen; Ekaterina Nevedomskaya; Alison Harrod; Roslin Russell; Judit Remenyi; Anna Maria Ochocka; Ross S. Thomas; Frances Fuller-Pace; Balázs Gyorffy; Carlos Caldas; Naveenan Navaratnam; Jason S. Carroll; Wilbert Zwart; R. Charles Coombes; Luca Magnani; Laki Buluwela; Simak Ali

doi:10.1016/j.celrep.2015.08.066

APOBEC3B-Mediated Cytidine Deamination Is Required for Estrogen Receptor Action in Breast Cancer

Manikandan Periyasamy
, Hetal Patel
, Chun Fui Lai
, Van T.M. Nguyen
, Ekaterina Nevedomskaya
, Alison Harrod
, Roslin Russell
, Judit Remenyi
, Anna Maria Ochocka
, Ross S. Thomas
, Frances Fuller-Pace
, Balázs Gyorffy
, Carlos Caldas
, Naveenan Navaratnam
, Jason S. Carroll
, Wilbert Zwart
, R. Charles Coombes
, Luca Magnani
, Laki Buluwela
, Simak Ali^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

98 Scopus citations

Abstract

Estrogen receptor α (ERα) is the key transcriptional driver in a large proportion of breast cancers. We report that APOBEC3B (A3B) is required for regulation of gene expression by ER and acts by causing C-to-U deamination at ER binding regions. We show that these C-to-U changes lead to the generation of DNA strand breaks through activation of base excision repair (BER) and to repair by non-homologous end-joining (NHEJ) pathways. We provide evidence that transient cytidine deamination by A3B aids chromatin modification and remodelling at the regulatory regions of ER target genes that promotes their expression. A3B expression is associated with poor patient survival in ER+ breast cancer, reinforcing the physiological significance of A3B for ER action.

Original language	English
Pages (from-to)	108-121
Number of pages	14
Journal	Cell Reports
Volume	13
Issue number	1
DOIs	https://doi.org/10.1016/j.celrep.2015.08.066
State	Published - 6 Oct 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 The Authors.

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1016/j.celrep.2015.08.066

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Periyasamy, M., Patel, H., Lai, C. F., Nguyen, V. T. M., Nevedomskaya, E., Harrod, A., Russell, R., Remenyi, J., Ochocka, A. M., Thomas, R. S., Fuller-Pace, F., Gyorffy, B., Caldas, C., Navaratnam, N., Carroll, J. S., Zwart, W., Coombes, R. C., Magnani, L., Buluwela, L., & Ali, S. (2015). APOBEC3B-Mediated Cytidine Deamination Is Required for Estrogen Receptor Action in Breast Cancer. Cell Reports, 13(1), 108-121. https://doi.org/10.1016/j.celrep.2015.08.066

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title = "APOBEC3B-Mediated Cytidine Deamination Is Required for Estrogen Receptor Action in Breast Cancer",

abstract = "Estrogen receptor α (ERα) is the key transcriptional driver in a large proportion of breast cancers. We report that APOBEC3B (A3B) is required for regulation of gene expression by ER and acts by causing C-to-U deamination at ER binding regions. We show that these C-to-U changes lead to the generation of DNA strand breaks through activation of base excision repair (BER) and to repair by non-homologous end-joining (NHEJ) pathways. We provide evidence that transient cytidine deamination by A3B aids chromatin modification and remodelling at the regulatory regions of ER target genes that promotes their expression. A3B expression is associated with poor patient survival in ER+ breast cancer, reinforcing the physiological significance of A3B for ER action.",

author = "Manikandan Periyasamy and Hetal Patel and Lai, \{Chun Fui\} and Nguyen, \{Van T.M.\} and Ekaterina Nevedomskaya and Alison Harrod and Roslin Russell and Judit Remenyi and Ochocka, \{Anna Maria\} and Thomas, \{Ross S.\} and Frances Fuller-Pace and Bal{\'a}zs Gyorffy and Carlos Caldas and Naveenan Navaratnam and Carroll, \{Jason S.\} and Wilbert Zwart and Coombes, \{R. Charles\} and Luca Magnani and Laki Buluwela and Simak Ali",

note = "Publisher Copyright: {\textcopyright} 2015 The Authors.",

year = "2015",

month = oct,

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doi = "10.1016/j.celrep.2015.08.066",

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Periyasamy, M, Patel, H, Lai, CF, Nguyen, VTM, Nevedomskaya, E, Harrod, A, Russell, R, Remenyi, J, Ochocka, AM, Thomas, RS, Fuller-Pace, F, Gyorffy, B, Caldas, C, Navaratnam, N, Carroll, JS, Zwart, W, Coombes, RC, Magnani, L, Buluwela, L & Ali, S 2015, 'APOBEC3B-Mediated Cytidine Deamination Is Required for Estrogen Receptor Action in Breast Cancer', Cell Reports, vol. 13, no. 1, pp. 108-121. https://doi.org/10.1016/j.celrep.2015.08.066

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T1 - APOBEC3B-Mediated Cytidine Deamination Is Required for Estrogen Receptor Action in Breast Cancer

AU - Periyasamy, Manikandan

AU - Patel, Hetal

AU - Lai, Chun Fui

AU - Nguyen, Van T.M.

AU - Nevedomskaya, Ekaterina

AU - Harrod, Alison

AU - Russell, Roslin

AU - Remenyi, Judit

AU - Ochocka, Anna Maria

AU - Thomas, Ross S.

AU - Fuller-Pace, Frances

AU - Gyorffy, Balázs

AU - Caldas, Carlos

AU - Navaratnam, Naveenan

AU - Carroll, Jason S.

AU - Zwart, Wilbert

AU - Coombes, R. Charles

AU - Magnani, Luca

AU - Buluwela, Laki

AU - Ali, Simak

PY - 2015/10/6

Y1 - 2015/10/6

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AB - Estrogen receptor α (ERα) is the key transcriptional driver in a large proportion of breast cancers. We report that APOBEC3B (A3B) is required for regulation of gene expression by ER and acts by causing C-to-U deamination at ER binding regions. We show that these C-to-U changes lead to the generation of DNA strand breaks through activation of base excision repair (BER) and to repair by non-homologous end-joining (NHEJ) pathways. We provide evidence that transient cytidine deamination by A3B aids chromatin modification and remodelling at the regulatory regions of ER target genes that promotes their expression. A3B expression is associated with poor patient survival in ER+ breast cancer, reinforcing the physiological significance of A3B for ER action.

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ER -

APOBEC3B-Mediated Cytidine Deamination Is Required for Estrogen Receptor Action in Breast Cancer

Abstract

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