TY - JOUR
T1 - Application of the Hybridization Chain Reaction on Electrodes for the Amplified and Parallel Electrochemical Analysis of DNA
AU - Trifonov, Alexander
AU - Sharon, Etery
AU - Tel-Vered, Ran
AU - Kahn, Jason S.
AU - Willner, Itamar
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/7/28
Y1 - 2016/7/28
N2 - The hybridization chain reaction (HCR) is implemented for the development of amplified electrochemical DNA sensing platforms. The target analyte hybridizes with a probe oligonucleotide-functionalized electrode and triggers on the HCR process in the presence of the hairpins HA and HB. The formation of the analyte-triggered HCR chains is followed by Faradaic impedance spectroscopy or chronocoulometry using Fe(CN)63-/4- or Ru(NH3)63+ as redox labels, respectively. By using two different probe-functionalized electrodes and a mixture of four hairpins, HA:HB and HC:HD, the parallel analysis of two analytes is demonstrated. Through the structural design of the hairpin structures to include caged G-quadruplex subunits, the analyte/probe hybrid associated with the electrode triggers on the HCR process, leading to G-quadruplex-functionalized HCR chains. The association of hemin to the matrix yields electrocatalytic hemin/G-quadruplex units that provide a secondary amplification path for the detection of DNA through an electrocatalyzed reduction of H2O2. The system allows the detection of the analyte DNA with a detection limit corresponding to 0.2 nM.
AB - The hybridization chain reaction (HCR) is implemented for the development of amplified electrochemical DNA sensing platforms. The target analyte hybridizes with a probe oligonucleotide-functionalized electrode and triggers on the HCR process in the presence of the hairpins HA and HB. The formation of the analyte-triggered HCR chains is followed by Faradaic impedance spectroscopy or chronocoulometry using Fe(CN)63-/4- or Ru(NH3)63+ as redox labels, respectively. By using two different probe-functionalized electrodes and a mixture of four hairpins, HA:HB and HC:HD, the parallel analysis of two analytes is demonstrated. Through the structural design of the hairpin structures to include caged G-quadruplex subunits, the analyte/probe hybrid associated with the electrode triggers on the HCR process, leading to G-quadruplex-functionalized HCR chains. The association of hemin to the matrix yields electrocatalytic hemin/G-quadruplex units that provide a secondary amplification path for the detection of DNA through an electrocatalyzed reduction of H2O2. The system allows the detection of the analyte DNA with a detection limit corresponding to 0.2 nM.
UR - http://www.scopus.com/inward/record.url?scp=84980027599&partnerID=8YFLogxK
U2 - 10.1021/acs.jpcc.5b11308
DO - 10.1021/acs.jpcc.5b11308
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AN - SCOPUS:84980027599
SN - 1932-7447
VL - 120
SP - 15743
EP - 15752
JO - Journal of Physical Chemistry C
JF - Journal of Physical Chemistry C
IS - 29
ER -