Application of the Hybridization Chain Reaction on Electrodes for the Amplified and Parallel Electrochemical Analysis of DNA

Alexander Trifonov, Etery Sharon, Ran Tel-Vered, Jason S. Kahn, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The hybridization chain reaction (HCR) is implemented for the development of amplified electrochemical DNA sensing platforms. The target analyte hybridizes with a probe oligonucleotide-functionalized electrode and triggers on the HCR process in the presence of the hairpins HA and HB. The formation of the analyte-triggered HCR chains is followed by Faradaic impedance spectroscopy or chronocoulometry using Fe(CN)63-/4- or Ru(NH3)63+ as redox labels, respectively. By using two different probe-functionalized electrodes and a mixture of four hairpins, HA:HB and HC:HD, the parallel analysis of two analytes is demonstrated. Through the structural design of the hairpin structures to include caged G-quadruplex subunits, the analyte/probe hybrid associated with the electrode triggers on the HCR process, leading to G-quadruplex-functionalized HCR chains. The association of hemin to the matrix yields electrocatalytic hemin/G-quadruplex units that provide a secondary amplification path for the detection of DNA through an electrocatalyzed reduction of H2O2. The system allows the detection of the analyte DNA with a detection limit corresponding to 0.2 nM.

Original languageEnglish
Pages (from-to)15743-15752
Number of pages10
JournalJournal of Physical Chemistry C
Volume120
Issue number29
DOIs
StatePublished - 28 Jul 2016

Bibliographical note

Publisher Copyright:
© 2016 American Chemical Society.

Fingerprint

Dive into the research topics of 'Application of the Hybridization Chain Reaction on Electrodes for the Amplified and Parallel Electrochemical Analysis of DNA'. Together they form a unique fingerprint.

Cite this