Ap4A Regulates Directional Mobility and Antigen Presentation in Dendritic Cells

Shin La Shu; Lakshmi Bhargavi Paruchuru; Neil Quanwei Tay; Yen Leong Chua; Adeline Shen Yun Foo; Chris Maolin Yang; Ka Hang Liong; Esther Geok Liang Koh; Angeline Lee; Hovav Nechushtan; Ehud Razin; David Michael Kemeny

doi:10.1016/j.isci.2019.05.045

Ap₄A Regulates Directional Mobility and Antigen Presentation in Dendritic Cells

Shin La Shu, Lakshmi Bhargavi Paruchuru, Neil Quanwei Tay, Yen Leong Chua, Adeline Shen Yun Foo, Chris Maolin Yang, Ka Hang Liong, Esther Geok Liang Koh, Angeline Lee, Hovav Nechushtan^*, Ehud Razin, David Michael Kemeny

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The significance of intracellular Ap₄A levels over immune activity of dendritic cells (DCs) has been studied in Nudt2^fl/fl/CD11c-cre mice. The transgenic mice have been generated by crossing floxed NUDT2 gene mice with DC marker CD11c recombinase (cre) mice. The DCs derived from these mice have higher levels of Ap₄A (≈30-fold) compared with those derived from Nudt2^+/+ mice. Interestingly, the elevated Ap₄A in DCs has led them to possess higher motility and lower directional variability. In addition, the DCs are able to enhance immune protection indicated by the higher cross-presentation of antigen and priming of CD8⁺ OT-I T cells. Overall, the study denotes prominent impact of Ap₄A over the functionality of DCs. The Nudt2^fl/fl/CD11c-cre mice could serve as a useful tool to study the influence of Ap₄A in the critical immune mechanisms of DCs.

Original language	English
Pages (from-to)	524-534
Number of pages	11
Journal	iScience
Volume	16
DOIs	https://doi.org/10.1016/j.isci.2019.05.045
State	Published - 28 Jun 2019

Bibliographical note

Publisher Copyright:
© 2019 The Author(s)

Keywords

Immune Response
Immunology
Model Organism
Molecular Mechanism of Behavior

Access to Document

10.1016/j.isci.2019.05.045

Cite this

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title = "Ap4A Regulates Directional Mobility and Antigen Presentation in Dendritic Cells",

abstract = "The significance of intracellular Ap4A levels over immune activity of dendritic cells (DCs) has been studied in Nudt2fl/fl/CD11c-cre mice. The transgenic mice have been generated by crossing floxed NUDT2 gene mice with DC marker CD11c recombinase (cre) mice. The DCs derived from these mice have higher levels of Ap4A (≈30-fold) compared with those derived from Nudt2+/+ mice. Interestingly, the elevated Ap4A in DCs has led them to possess higher motility and lower directional variability. In addition, the DCs are able to enhance immune protection indicated by the higher cross-presentation of antigen and priming of CD8+ OT-I T cells. Overall, the study denotes prominent impact of Ap4A over the functionality of DCs. The Nudt2fl/fl/CD11c-cre mice could serve as a useful tool to study the influence of Ap4A in the critical immune mechanisms of DCs.",

keywords = "Immune Response, Immunology, Model Organism, Molecular Mechanism of Behavior",

author = "{La Shu}, Shin and Paruchuru, {Lakshmi Bhargavi} and Tay, {Neil Quanwei} and Chua, {Yen Leong} and {Yun Foo}, {Adeline Shen} and Yang, {Chris Maolin} and Liong, {Ka Hang} and {Liang Koh}, {Esther Geok} and Angeline Lee and Hovav Nechushtan and Ehud Razin and Kemeny, {David Michael}",

note = "Publisher Copyright: {\textcopyright} 2019 The Author(s)",

year = "2019",

month = jun,

day = "28",

doi = "10.1016/j.isci.2019.05.045",

language = "אנגלית",

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AU - La Shu, Shin

AU - Paruchuru, Lakshmi Bhargavi

AU - Tay, Neil Quanwei

AU - Chua, Yen Leong

AU - Yun Foo, Adeline Shen

AU - Yang, Chris Maolin

AU - Liong, Ka Hang

AU - Liang Koh, Esther Geok

AU - Lee, Angeline

AU - Nechushtan, Hovav

AU - Razin, Ehud

AU - Kemeny, David Michael

PY - 2019/6/28

Y1 - 2019/6/28

N2 - The significance of intracellular Ap4A levels over immune activity of dendritic cells (DCs) has been studied in Nudt2fl/fl/CD11c-cre mice. The transgenic mice have been generated by crossing floxed NUDT2 gene mice with DC marker CD11c recombinase (cre) mice. The DCs derived from these mice have higher levels of Ap4A (≈30-fold) compared with those derived from Nudt2+/+ mice. Interestingly, the elevated Ap4A in DCs has led them to possess higher motility and lower directional variability. In addition, the DCs are able to enhance immune protection indicated by the higher cross-presentation of antigen and priming of CD8+ OT-I T cells. Overall, the study denotes prominent impact of Ap4A over the functionality of DCs. The Nudt2fl/fl/CD11c-cre mice could serve as a useful tool to study the influence of Ap4A in the critical immune mechanisms of DCs.

AB - The significance of intracellular Ap4A levels over immune activity of dendritic cells (DCs) has been studied in Nudt2fl/fl/CD11c-cre mice. The transgenic mice have been generated by crossing floxed NUDT2 gene mice with DC marker CD11c recombinase (cre) mice. The DCs derived from these mice have higher levels of Ap4A (≈30-fold) compared with those derived from Nudt2+/+ mice. Interestingly, the elevated Ap4A in DCs has led them to possess higher motility and lower directional variability. In addition, the DCs are able to enhance immune protection indicated by the higher cross-presentation of antigen and priming of CD8+ OT-I T cells. Overall, the study denotes prominent impact of Ap4A over the functionality of DCs. The Nudt2fl/fl/CD11c-cre mice could serve as a useful tool to study the influence of Ap4A in the critical immune mechanisms of DCs.

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