Arabidopsis thaliana endo-1,4-β-glucanase (cel1) promoter mediates uidA expression in elongating tissues of aspen (Populus tremula)

Ziv Shani, Mara Dekel, Christian Sig Jensen, Tzvi Tzfira, Raphael Goren, Arie Altman, Oded Shoseyov*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Transgenic aspen plants were transformed with a bacterial β-glucuronidase gene (uidA) under the control of the Arabidopsis thaliana elongation-specific endo-1,4-β-glucanase (cel1) promoter. Transformed plants exhibited a high level of β-glucuronidase (GUS) activity in young, fast-growing tissues. Unlike the cauliflower mosaique virus 35S promoter, the expression pattern of uidA in the shoots and leaves was dear-cut and highly controlled by cel1 promoter. GUS-staining in the leaves mirrored the pattern of expanding cells in the organ. These results further substantiate the link between cel1 expression and plant cell elongation. Moreover, our results suggest that the cell promoter may be useful in the genetic engineering of forest trees and possibly other plants when gene expression is desired only in fast growing tissues.

Original languageAmerican English
Pages (from-to)118-120
Number of pages3
JournalJournal of Plant Physiology
Volume156
Issue number1
DOIs
StatePublished - Jan 2000

Keywords

  • Cellulase
  • Elongation
  • Endo-1,4-β-glucanase
  • Forest tree
  • Poplar
  • Promoter

Fingerprint

Dive into the research topics of 'Arabidopsis thaliana endo-1,4-β-glucanase (cel1) promoter mediates uidA expression in elongating tissues of aspen (Populus tremula)'. Together they form a unique fingerprint.

Cite this