Archaea in the Gulf of Aqaba

Danny Ionescu*, Sigrid Penno, Maya Haimovich, Branko Rihtman, Aram Goodwin, Daniel Schwartz, Lena Hazanov, Mark Chernihovsky, Anton F. Post, Aharon Oren

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Using a polyphasic approach, we examined the presence of Archaea in the Gulf of Aqaba, a warm marine ecosystem, isolated from major ocean currents and subject to pronounced seasonal changes in hydrography. Catalyzed reported deposition FISH analyses showed that Archaea make up to >20% of the prokaryotic community in the Gulf. A spatial separation between the two major phyla of Archaea was observed during summer stratification. Euryarchaeota were found exclusively in the upper 200 m, whereas Crenarchaeota were present in greater numbers in layers below the summer thermocline. 16S rRNA gene-based denaturing gradient gel electrophoresis confirmed this depth partitioning and revealed further diversity of Crenarchaeota and Euryarchaeota populations along depth profiles. Phylogenetic analysis showed pelagic Crenarchaeota and Euryarchaeota to differ from coral-associated Archaea from the Gulf, forming distinct clusters within the Marine Archaea Groups I and II. Endsequencing of fosmid libraries of environmental DNA provided a tentative identification of some members of the archaeal community and their role in the microbial community of the Gulf. Incorporation studies of radiolabeled leucine and bicarbonate in the presence of different inhibitors suggest that the archaeal community participates in autotrophic CO2 uptake and contributes little to the heterotrophic activity.

Original languageAmerican English
Pages (from-to)425-438
Number of pages14
JournalFEMS Microbiology Ecology
Volume69
Issue number3
DOIs
StatePublished - Sep 2009

Keywords

  • Archaea
  • Crenarchaeota
  • Euryarchaeota
  • Gulf of Aqaba
  • Inorganic CO uptake

Fingerprint

Dive into the research topics of 'Archaea in the Gulf of Aqaba'. Together they form a unique fingerprint.

Cite this