TY - JOUR
T1 - Architecture of an RNA polymerase II transcription pre-initiation complex
AU - Murakami, Kenji
AU - Elmlund, Hans
AU - Kalisman, Nir
AU - Bushnell, David A.
AU - Adams, Christopher M.
AU - Azubel, Maia
AU - Elmlund, Dominika
AU - Levi-Kalisman, Yael
AU - Liu, Xin
AU - Gibbons, Brian J.
AU - Levitt, Michael
AU - Kornberg, Roger D.
PY - 2013
Y1 - 2013
N2 - The protein density and arrangement of subunits of a complete, 32-protein, RNA polymerase II (pol II) transcription pre-initiation complex (PIC) were determined by means of cryogenic electron microscopy and a combination of chemical cross-linking and mass spectrometry. The PIC showed a marked division in two parts, one containing all the general transcription factors (GTFs) and the other pol II. Promoter DNA was associated only with the GTFs, suspended above the pol II cleft and not in contact with pol II. This structural principle of the PIC underlies its conversion to a transcriptionally active state; the PIC is poised for the formation of a transcription bubble and descent of the DNA into the pol II cleft.
AB - The protein density and arrangement of subunits of a complete, 32-protein, RNA polymerase II (pol II) transcription pre-initiation complex (PIC) were determined by means of cryogenic electron microscopy and a combination of chemical cross-linking and mass spectrometry. The PIC showed a marked division in two parts, one containing all the general transcription factors (GTFs) and the other pol II. Promoter DNA was associated only with the GTFs, suspended above the pol II cleft and not in contact with pol II. This structural principle of the PIC underlies its conversion to a transcriptionally active state; the PIC is poised for the formation of a transcription bubble and descent of the DNA into the pol II cleft.
UR - http://www.scopus.com/inward/record.url?scp=84884516365&partnerID=8YFLogxK
U2 - 10.1126/science.1238724
DO - 10.1126/science.1238724
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C2 - 24072820
AN - SCOPUS:84884516365
SN - 0036-8075
VL - 342
JO - Science
JF - Science
IS - 6159
M1 - 1238724
ER -