Abstract
Methyl methane sulphonate (MMS) was found to arrest mitotic cells at a specific stage in the cell cycle. Reciprocal double shift experiments involving MMS and temperature shifts in several temperature-sensitive cell-cycle (cdc) mutants have located the MMS-sensitive stage after the cdc7 and cdc8 temperature-sensitive stages and before the cdc13, cdc5 and cdc14 stages. An interdependent relationship was found between the arrests caused by MMS, cdc40 and hydroxyurea. Marked increases in mitotic recombination were induced by MMS, both in diploid and haploid strains. Meiosis is arrested by MMS at a very early stage, before DNA replication.
| Original language | English |
|---|---|
| Pages (from-to) | 558-564 |
| Number of pages | 7 |
| Journal | Zeitschrift fur Vererbungslehre |
| Volume | 201 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 1985 |