TY - JOUR
T1 - Assemblies of psoriatic keratin and their relation to normal intermediate filament structures
AU - Trachtenberg, Shlomo
PY - 1987/3/19
Y1 - 1987/3/19
N2 - Protein extracts from normal human epidermis reassemble in vitro into 8-10 nm diameter filaments characteristic of intermediate filaments, whereas extracts from psoriatic epidermal scales reassemble, under identical conditions, into a variety of paracrystalline bundles. Optical diffraction and image analysis of these paracrystalline bundles reveal an axial repeat of 16.5 nm, which subdivides into three bands of 5.5 nm, and a lateral spacing of 5.1 nm. This information, together with available sequence studies of intermediate filaments and biochemical data, suggests that the subunit of psoriatic keratin is made up essentially from the coiled-coil α-helical rod domain of the normal keratin subunits, whereas the random coil domains are missing or greatly reduced in size.
AB - Protein extracts from normal human epidermis reassemble in vitro into 8-10 nm diameter filaments characteristic of intermediate filaments, whereas extracts from psoriatic epidermal scales reassemble, under identical conditions, into a variety of paracrystalline bundles. Optical diffraction and image analysis of these paracrystalline bundles reveal an axial repeat of 16.5 nm, which subdivides into three bands of 5.5 nm, and a lateral spacing of 5.1 nm. This information, together with available sequence studies of intermediate filaments and biochemical data, suggests that the subunit of psoriatic keratin is made up essentially from the coiled-coil α-helical rod domain of the normal keratin subunits, whereas the random coil domains are missing or greatly reduced in size.
KW - Image analysis
KW - Intermediate filament
KW - Keratin
KW - Psoriasis
UR - http://www.scopus.com/inward/record.url?scp=0023130335&partnerID=8YFLogxK
U2 - 10.1016/0304-4165(87)90039-0
DO - 10.1016/0304-4165(87)90039-0
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C2 - 2435324
AN - SCOPUS:0023130335
SN - 0304-4165
VL - 923
SP - 327
EP - 332
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
IS - 3
ER -