Assembly and processing of subunit II (PsaD) precursor in the isolated photosystem-I complex

Yuval Cohen; Rachel Nechushtai

doi:10.1016/0014-5793(92)80273-J

Assembly and processing of subunit II (PsaD) precursor in the isolated photosystem-I complex

Yuval Cohen, Rachel Nechushtai^*

^*Corresponding author for this work

Department of Plant and Environmental Sciences (Natural Sciences)

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The precursor of photosystem I (PSI) subunit II (pre-subunit II) synthesized in vitro, was found to bind to the holo-PSI complex, both within the thylakoids and outside, after detergent extraction of PSI from the membranes. Chloroplast stromal fraction added to the purified PSI complexes, containing the labeled pre-subunit II, induced the processing of the precursor to the mature form. This implies that processing can occur within the isolated complex, after the integration of the precursor. The results presented suggest that certain aspects of biogenesis of membranal protein complexes can be studied in detergent-extracted purified complexes.

Original language	English
Pages (from-to)	15-17
Number of pages	3
Journal	FEBS Letters
Volume	302
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(92)80273-J
State	Published - 4 May 1992

Bibliographical note

Funding Information:
Al,kr~nn,lc~r/T~hics rw,rorr~k ~wars/ .ssu:p ported by BSF grams (NO. 87-00019a nd 8%00360), and by a research grant from Charles H. Rcvson Foundnlion (No. 828/f%), WC wish IO thank Prol’. 1. Larch for helpful discussions and suggcslions.

Keywords

Assembly
Biogenesis
Photosystem I
Processing
PsaD

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10.1016/0014-5793(92)80273-J

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title = "Assembly and processing of subunit II (PsaD) precursor in the isolated photosystem-I complex",

abstract = "The precursor of photosystem I (PSI) subunit II (pre-subunit II) synthesized in vitro, was found to bind to the holo-PSI complex, both within the thylakoids and outside, after detergent extraction of PSI from the membranes. Chloroplast stromal fraction added to the purified PSI complexes, containing the labeled pre-subunit II, induced the processing of the precursor to the mature form. This implies that processing can occur within the isolated complex, after the integration of the precursor. The results presented suggest that certain aspects of biogenesis of membranal protein complexes can be studied in detergent-extracted purified complexes.",

keywords = "Assembly, Biogenesis, Photosystem I, Processing, PsaD",

author = "Yuval Cohen and Rachel Nechushtai",

note = "Funding Information: Al,kr~nn,lc~r/T~hics rw,rorr~k ~wars/ .ssu:p ported by BSF grams (NO. 87-00019a nd 8%00360), and by a research grant from Charles H. Rcvson Foundnlion (No. 828/f%), WC wish IO thank Prol{\textquoteright}. 1. Larch for helpful discussions and suggcslions.",

year = "1992",

month = may,

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doi = "10.1016/0014-5793(92)80273-J",

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AU - Cohen, Yuval

AU - Nechushtai, Rachel

N1 - Funding Information: Al,kr~nn,lc~r/T~hics rw,rorr~k ~wars/ .ssu:p ported by BSF grams (NO. 87-00019a nd 8%00360), and by a research grant from Charles H. Rcvson Foundnlion (No. 828/f%), WC wish IO thank Prol’. 1. Larch for helpful discussions and suggcslions.

PY - 1992/5/4

Y1 - 1992/5/4

N2 - The precursor of photosystem I (PSI) subunit II (pre-subunit II) synthesized in vitro, was found to bind to the holo-PSI complex, both within the thylakoids and outside, after detergent extraction of PSI from the membranes. Chloroplast stromal fraction added to the purified PSI complexes, containing the labeled pre-subunit II, induced the processing of the precursor to the mature form. This implies that processing can occur within the isolated complex, after the integration of the precursor. The results presented suggest that certain aspects of biogenesis of membranal protein complexes can be studied in detergent-extracted purified complexes.

AB - The precursor of photosystem I (PSI) subunit II (pre-subunit II) synthesized in vitro, was found to bind to the holo-PSI complex, both within the thylakoids and outside, after detergent extraction of PSI from the membranes. Chloroplast stromal fraction added to the purified PSI complexes, containing the labeled pre-subunit II, induced the processing of the precursor to the mature form. This implies that processing can occur within the isolated complex, after the integration of the precursor. The results presented suggest that certain aspects of biogenesis of membranal protein complexes can be studied in detergent-extracted purified complexes.

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KW - Biogenesis

KW - Photosystem I

KW - Processing

KW - PsaD

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ER -

Assembly and processing of subunit II (PsaD) precursor in the isolated photosystem-I complex

Abstract

Bibliographical note

Keywords

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