Assembly and processing of subunit II (PsaD) precursor in the isolated photosystem-I complex

Yuval Cohen, Rachel Nechushtai*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The precursor of photosystem I (PSI) subunit II (pre-subunit II) synthesized in vitro, was found to bind to the holo-PSI complex, both within the thylakoids and outside, after detergent extraction of PSI from the membranes. Chloroplast stromal fraction added to the purified PSI complexes, containing the labeled pre-subunit II, induced the processing of the precursor to the mature form. This implies that processing can occur within the isolated complex, after the integration of the precursor. The results presented suggest that certain aspects of biogenesis of membranal protein complexes can be studied in detergent-extracted purified complexes.

Original languageAmerican English
Pages (from-to)15-17
Number of pages3
JournalFEBS Letters
Issue number1
StatePublished - 4 May 1992

Bibliographical note

Funding Information:
Al,kr~nn,lc~r/T~hics rw,rorr~k ~wars/ .ssu:p ported by BSF grams (NO. 87-00019a nd 8%00360), and by a research grant from Charles H. Rcvson Foundnlion (No. 828/f%), WC wish IO thank Prol’. 1. Larch for helpful discussions and suggcslions.


  • Assembly
  • Biogenesis
  • Photosystem I
  • Processing
  • PsaD


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