Assembly of the SpoIIIE DNA Translocase Depends on Chromosome Trapping in Bacillus subtilis

Sigal Ben-Yehuda, David Z. Rudner, Richard Losick*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Sporulation in Bacillus subtilis is an attractive system in which to study the translocation of a chromosome across a membrane. Sporulating cells contain two sister chromosomes that are condensed in an elongated axial filament with the origins of replication anchored at opposite poles of the sporangium [1,2]. The subsequent formation of a septum near one pole divides the sporangium unequally into a forespore (the smaller compartment) and a mother cell [3]. The septum forms around the filament, trapping the origin-proximal region of one chromosome in the forespore. As a consequence, the trapped chromosome transverses the septum with the remainder being left in the mother cell [4]. Next, SpoIIIE assembles at the middle of the septum to create a translocase that pumps the origin-distal, two-thirds of the chromosome into the forespore [5]. Here, we address the question of how the DNA translocase assembles and how it localizes to the septal midpoint. We present evidence that DNA transversing the septum is an anchor that nucleates the formation of the DNA translocase. We propose that DNA anchoring is responsible for the assembly of other SpoIIIE-like DNA translocases, such as those that remove trapped chromosomes from the division septum of cells undergoing binary fission.

Original languageAmerican English
Pages (from-to)2196-2200
Number of pages5
JournalCurrent Biology
Volume13
Issue number24
DOIs
StatePublished - 16 Dec 2003

Bibliographical note

Funding Information:
We thank E. González-Pastor for providing the SpoIIIE-GFP fusion and members of the Losick laboratory for comments on the manuscript. We also thank R. Helmiss for assistance with computer graphics. S.B.-Y. is a postdoctoral fellow of the Human Frontier Science Program. This work was supported by NIH grant GM18568 to R.L.

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