Assessing Pseudomonas aeruginosa persister/antibiotic tolerant cells

Ronen Hazan, Damien Maura, Yok Ai Que, Laurence G. Rahme

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Bacterial persistence, which is observed in a broad range of microbial species, is the capacity of a bacterial cell subpopulation called “persisters” to tolerate exposure to normally lethal concentrations of bactericidal antibiotics. This ability, which is not due to antibiotic-resistant mutants, has been implicated in antibiotic treatment failures and may account for latent, chronic, and relapsing infections. Antibiotic tolerant/Persister (AT/P) cells have been notoriously difficult to study due to their low frequency and transient nature. This chapter describes the main methods used to isolate and study Pseudomonas aeruginosa AT/P cells and discusses new technologies that may ease research of P. aeruginosa persisters in the near future.

Original languageAmerican English
Pages (from-to)699-707
Number of pages9
JournalMethods in Molecular Biology
Volume1149
DOIs
StatePublished - 2014

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2014.

Keywords

  • Anti-infectives
  • Anti-virulence
  • Antibiotic tolerance
  • Antibiotics
  • Bacterial persistence
  • High-throughput screen
  • Infection
  • Proliferation
  • Quorum sensing
  • Small molecules
  • Virulence

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