Association between DNA markers and loci controlling avocado traits

Dror Sharon, Jossi Hillel, Samir Mhameed, Perry B. Cregan, Emanuel Lahav, Uri Lavi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The detection of association between DNA markers and traits of interest in an outbred population is complicated and requires highly polymorphic markers. A genetic linkage map of avocado (Persea americana Mill.) recently generated consists of simple sequence repeat (SSR) markers as well as DNA fingerprint (DFP) and randomly amplified polymorphic DNA (RAPD) markers. These markers were used to detect putative quantitative trait loci (QTLs) of eight avocado fruit traits. Two statistical methods were used: one-way analysis of variance and interval mapping. Six traits were found to be associated with at least one of the 90 DNA markers. Based on the two statistical approaches, a putative QTL associated with the presence of fibers in the flesh, was found to be located on linkage group 3. This putative QTL was found to be associated with the SSR marker AVA04 having a high significant value (P = 4.4 x 10-8). The haplotype analysis of linkage group 3 showed a putative dominant interaction between the alleles of this locus.

Original languageEnglish
Pages (from-to)1016-1022
Number of pages7
JournalJournal of the American Society for Horticultural Science
Volume123
Issue number6
DOIs
StatePublished - Nov 1998

Keywords

  • DNA fingerprints
  • Genetic association
  • Interval mapping
  • Microsatellites

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