Association between IL-10a single nucleotide polymorphisms and resistance to cyprinid herpesvirus-3 infection in common carp (Cyprinus carpio)

Pawapol Kongchum; Eran Sandel; Sigal Lutzky; Eric M. Hallerman; Gideon Hulata; Lior David; Yniv Palti

doi:10.1016/j.aquaculture.2011.02.035

Association between IL-10a single nucleotide polymorphisms and resistance to cyprinid herpesvirus-3 infection in common carp (Cyprinus carpio)

Pawapol Kongchum, Eran Sandel, Sigal Lutzky, Eric M. Hallerman, Gideon Hulata, Lior David, Yniv Palti^*

^*Corresponding author for this work

Department of Animal Sciences

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

Analysis of gene polymorphisms and disease association is essential for assessing putative candidate genes affecting susceptibility or resistance to disease. In this paper, we report the results of an association analysis between single nucleotide polymorphisms (SNPs) in common carp (Cyprinus carpio) innate immune response genes and resistance to cyprinid herpesvirus. -3 (CyHV. -3) infection. Twenty. -three SNPs from 14 genes were genotyped in the progeny of a full. -sib family, which was subjected to a CyHV. -3 challenge. The challenge was carried out by cohabitation of naïve fish with CyHV. -3. -infected fish and the experimental fish subsequently were scored as susceptible or resistant. Fisher exact probability and chi. -square tests were used to assess the association of SNP genotypes or alleles with CyHV. -3 resistance. No association between SNPs in common carp immune response genes and resistance to CyHV. -3 was found, except for SNPs in IL-10a. For IL-10a, both heterozygous and homozygous fish carrying the GCG haplotype were more susceptible to CyHV. -3 infection (P<. 0.001). To identify the source of susceptible and resistant alleles, we genotyped fish from the parental common carp strains. We found that the susceptible allele was more common among fish from the wild CyHV. -3. -resistant strain (Sassan) whereas the resistant allele was found in all the fish sampled from the susceptible domesticated strain (Našice). The implications of our findings to the development of a domesticated CyHV. -3. -resistant stock through marker. -assisted selection or gene. -introgression, and the possible impact of the IL-10a polymorphisms we identified on susceptibility to CyHV. -3 infection in common carp are discussed.

Original language	English
Pages (from-to)	417-421
Number of pages	5
Journal	Aquaculture
Volume	315
Issue number	3-4
DOIs	https://doi.org/10.1016/j.aquaculture.2011.02.035
State	Published - 21 May 2011

Bibliographical note

Funding Information:
This study was supported by a grant from the US–Israel Binational Agricultural Research and Development Fund (BARD Project No. IS-3997-07 ). P.K. was also supported by a Royal Thai Government doctoral scholarship. E.H. is supported by Virginia Agricultural Experimental Station through the USDA-NIFA Hatch Program. We thank Kristy Shewbridge, Roseanna Long and Brian Smith for their technical assistance. We also thank Dr. Roger Vallejo and Dr. Gregory Wiens for their help and guidance with data analysis and protein modeling, and Dr. Maureen Purcell for reviewing the manuscript draft. The mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.

Keywords

Common carp, Cyprinus carpio
Cyprinid herpesvirus-3
Disease resistance
Innate immune response
Marker-assisted breeding
Single nucleotide polymorphisms

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10.1016/j.aquaculture.2011.02.035

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title = "Association between IL-10a single nucleotide polymorphisms and resistance to cyprinid herpesvirus-3 infection in common carp (Cyprinus carpio)",

abstract = "Analysis of gene polymorphisms and disease association is essential for assessing putative candidate genes affecting susceptibility or resistance to disease. In this paper, we report the results of an association analysis between single nucleotide polymorphisms (SNPs) in common carp (Cyprinus carpio) innate immune response genes and resistance to cyprinid herpesvirus. -3 (CyHV. -3) infection. Twenty. -three SNPs from 14 genes were genotyped in the progeny of a full. -sib family, which was subjected to a CyHV. -3 challenge. The challenge was carried out by cohabitation of na{\"i}ve fish with CyHV. -3. -infected fish and the experimental fish subsequently were scored as susceptible or resistant. Fisher exact probability and chi. -square tests were used to assess the association of SNP genotypes or alleles with CyHV. -3 resistance. No association between SNPs in common carp immune response genes and resistance to CyHV. -3 was found, except for SNPs in IL-10a. For IL-10a, both heterozygous and homozygous fish carrying the GCG haplotype were more susceptible to CyHV. -3 infection (P<. 0.001). To identify the source of susceptible and resistant alleles, we genotyped fish from the parental common carp strains. We found that the susceptible allele was more common among fish from the wild CyHV. -3. -resistant strain (Sassan) whereas the resistant allele was found in all the fish sampled from the susceptible domesticated strain (Na{\v s}ice). The implications of our findings to the development of a domesticated CyHV. -3. -resistant stock through marker. -assisted selection or gene. -introgression, and the possible impact of the IL-10a polymorphisms we identified on susceptibility to CyHV. -3 infection in common carp are discussed.",

keywords = "Common carp, Cyprinus carpio, Cyprinid herpesvirus-3, Disease resistance, Innate immune response, Marker-assisted breeding, Single nucleotide polymorphisms",

author = "Pawapol Kongchum and Eran Sandel and Sigal Lutzky and Hallerman, {Eric M.} and Gideon Hulata and Lior David and Yniv Palti",

note = "Funding Information: This study was supported by a grant from the US–Israel Binational Agricultural Research and Development Fund (BARD Project No. IS-3997-07 ). P.K. was also supported by a Royal Thai Government doctoral scholarship. E.H. is supported by Virginia Agricultural Experimental Station through the USDA-NIFA Hatch Program. We thank Kristy Shewbridge, Roseanna Long and Brian Smith for their technical assistance. We also thank Dr. Roger Vallejo and Dr. Gregory Wiens for their help and guidance with data analysis and protein modeling, and Dr. Maureen Purcell for reviewing the manuscript draft. The mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.",

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AU - Kongchum, Pawapol

AU - Sandel, Eran

AU - Lutzky, Sigal

AU - Hallerman, Eric M.

AU - Hulata, Gideon

AU - David, Lior

AU - Palti, Yniv

N1 - Funding Information: This study was supported by a grant from the US–Israel Binational Agricultural Research and Development Fund (BARD Project No. IS-3997-07 ). P.K. was also supported by a Royal Thai Government doctoral scholarship. E.H. is supported by Virginia Agricultural Experimental Station through the USDA-NIFA Hatch Program. We thank Kristy Shewbridge, Roseanna Long and Brian Smith for their technical assistance. We also thank Dr. Roger Vallejo and Dr. Gregory Wiens for their help and guidance with data analysis and protein modeling, and Dr. Maureen Purcell for reviewing the manuscript draft. The mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.

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N2 - Analysis of gene polymorphisms and disease association is essential for assessing putative candidate genes affecting susceptibility or resistance to disease. In this paper, we report the results of an association analysis between single nucleotide polymorphisms (SNPs) in common carp (Cyprinus carpio) innate immune response genes and resistance to cyprinid herpesvirus. -3 (CyHV. -3) infection. Twenty. -three SNPs from 14 genes were genotyped in the progeny of a full. -sib family, which was subjected to a CyHV. -3 challenge. The challenge was carried out by cohabitation of naïve fish with CyHV. -3. -infected fish and the experimental fish subsequently were scored as susceptible or resistant. Fisher exact probability and chi. -square tests were used to assess the association of SNP genotypes or alleles with CyHV. -3 resistance. No association between SNPs in common carp immune response genes and resistance to CyHV. -3 was found, except for SNPs in IL-10a. For IL-10a, both heterozygous and homozygous fish carrying the GCG haplotype were more susceptible to CyHV. -3 infection (P<. 0.001). To identify the source of susceptible and resistant alleles, we genotyped fish from the parental common carp strains. We found that the susceptible allele was more common among fish from the wild CyHV. -3. -resistant strain (Sassan) whereas the resistant allele was found in all the fish sampled from the susceptible domesticated strain (Našice). The implications of our findings to the development of a domesticated CyHV. -3. -resistant stock through marker. -assisted selection or gene. -introgression, and the possible impact of the IL-10a polymorphisms we identified on susceptibility to CyHV. -3 infection in common carp are discussed.

AB - Analysis of gene polymorphisms and disease association is essential for assessing putative candidate genes affecting susceptibility or resistance to disease. In this paper, we report the results of an association analysis between single nucleotide polymorphisms (SNPs) in common carp (Cyprinus carpio) innate immune response genes and resistance to cyprinid herpesvirus. -3 (CyHV. -3) infection. Twenty. -three SNPs from 14 genes were genotyped in the progeny of a full. -sib family, which was subjected to a CyHV. -3 challenge. The challenge was carried out by cohabitation of naïve fish with CyHV. -3. -infected fish and the experimental fish subsequently were scored as susceptible or resistant. Fisher exact probability and chi. -square tests were used to assess the association of SNP genotypes or alleles with CyHV. -3 resistance. No association between SNPs in common carp immune response genes and resistance to CyHV. -3 was found, except for SNPs in IL-10a. For IL-10a, both heterozygous and homozygous fish carrying the GCG haplotype were more susceptible to CyHV. -3 infection (P<. 0.001). To identify the source of susceptible and resistant alleles, we genotyped fish from the parental common carp strains. We found that the susceptible allele was more common among fish from the wild CyHV. -3. -resistant strain (Sassan) whereas the resistant allele was found in all the fish sampled from the susceptible domesticated strain (Našice). The implications of our findings to the development of a domesticated CyHV. -3. -resistant stock through marker. -assisted selection or gene. -introgression, and the possible impact of the IL-10a polymorphisms we identified on susceptibility to CyHV. -3 infection in common carp are discussed.

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KW - Cyprinid herpesvirus-3

KW - Disease resistance

KW - Innate immune response

KW - Marker-assisted breeding

KW - Single nucleotide polymorphisms

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Association between IL-10a single nucleotide polymorphisms and resistance to cyprinid herpesvirus-3 infection in common carp (Cyprinus carpio)

Abstract

Bibliographical note

Keywords

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