Association between the cell cycle and neural crest delamination through specific regulation of G1/S transition

Tal Burstyn-Cohen*, Chaya Kalcheim

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

109 Scopus citations

Abstract

Delamination of premigratory neural crest cells from the dorsal neural tube depends both upon environmental signals and cell-intrinsic mechanisms and is a prerequisite for cells to engage in migration. Here we show that avian neural crest cells synchronously emigrate from the neural tube in the S phase of the cell cycle. Furthermore, specific inhibition of the transition from G1 to S both in ovo and in explants blocks delamination, whereas arrest at the S or G2 phases has no immediate effect. Thus, the events taking place during G1 that control the transition from G1 to S are necessary for the epithelial to mesenchymal conversion of crest precursors.

Original languageAmerican English
Pages (from-to)383-395
Number of pages13
JournalDevelopmental Cell
Volume3
Issue number3
DOIs
StatePublished - 1 Sep 2002

Bibliographical note

Funding Information:
We thank all members of our group for discussions, in particular, Yuval Cinnamon and Raz Ben-Yair for assistance with figures and cell counts. We also thank J. Yisraeli and B. Paterson for critical reading of the manuscript. We are indebted to B. Paterson, D. Ginsberg, M. Brandeis, and T. Momose for the 15 aa-MyoD-GFP, dNE2F-1, p27, and GFP expression vectors, respectively. A. Levitzki and A. Gazit kindly provided us AG555 and AG18. This work was supported by grants from the March of Dimes, ICRF, and DFG (SFB 488) to Chaya Kalcheim. T.B.-C. was supported by a postdoctoral fellowship from the ICRF.

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