Asynchronous replication of imprinted genes is established in the gametes and maintained during development

Itamar Simon, Toyoaki Tenzen, Benjamin E. Reubinoff, Dahlia Hillman, John R. McCarrey, Howard Cedar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

143 Scopus citations

Abstract

Genomic imprinting is characterized by allele-specific expression of multiple genes within large chromosomal domains that undergo DNA replication asynchronously during S phase. Here we show, using both fluorescence in situ hybridization analysis and S-phase fractionation techniques, that differential replication timing is associated with imprinted genes in a variety of cell types, and is already present in the pre-implantation embryo soon after fertilization. This pattern is erased before meiosis in the germ line, and parent-specific replication timing is then reset in late gametogenesis in both the male and female. Thus, asynchronous replication timing is established in the gametes and maintained throughout development, indicating that it may function as a primary epigenetic marker for distinguishing between the parental alleles.

Original languageAmerican English
Pages (from-to)929-932
Number of pages4
JournalNature
Volume401
Issue number6756
DOIs
StatePublished - 28 Oct 1999

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