ATP-Responsive Aptamer-Based Metal–Organic Framework Nanoparticles (NMOFs) for the Controlled Release of Loads and Drugs

Wei Hai Chen, Xu Yu, Wei Ching Liao, Yang Sung Sohn, Alessandro Cecconello, Anna Kozell, Rachel Nechushtai, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

167 Scopus citations

Abstract

Nanoparticles consisting of metal–organic frameworks (NMOFs) modified with nucleic acid binding strands are synthesized. The NMOFs are loaded with a fluorescent agent or with the anticancer drug doxorubicin, and the loaded NMOFs are capped by hybridization with a complementary nucleic acid that includes the ATP-aptamer or the ATP-AS1411 hybrid aptamer in caged configurations. The NMOFs are unlocked in the presence of ATP via the formation of ATP-aptamer complexes, resulting in the release of the loads. As ATP is overexpressed in cancer cells, and since the AS1411 aptamer recognizes the nucleolin receptor sites on the cancer cell membrane, the doxorubicin-loaded NMOFs provide functional carriers for targeting and treatment of cancer cells. Preliminary cell experiments reveal impressive selective permeation of the NMOFs into MDA-MB-231 breast cancer cells as compared to MCF-10A normal epithelial breast cells. High cytotoxic efficacy and targeted drug release are observed with the ATP-AS1411-functionalized doxorubicin-loaded NMOFs.

Original languageAmerican English
Article number1702102
JournalAdvanced Functional Materials
Volume27
Issue number37
DOIs
StatePublished - 5 Oct 2017

Bibliographical note

Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

  • cytotoxicity
  • doxorubicin
  • drug release
  • nucleic acids
  • targeting

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