TY - JOUR
T1 - Attenuation of food intake in chicks by an inverse agonist of cannabinoid receptor 1 administered by either injection or ingestion in hydrocolloid carriers
AU - Novoseletsky, Nataly
AU - Nussinovitch, Amos
AU - Friedman-Einat, Miriam
PY - 2011/2/1
Y1 - 2011/2/1
N2 - The cannabinoid receptor (CB1) was studied primarily in mammals where it was found to comprise a link between reward processes and addictive behavior such as food consumption. The purpose of this study was twofold: first to characterize the effect of the chicken CB1 receptor inverse agonist AM251 on food intake, and second, to establish a stress-free approach for application of AM251 to birds using hydrocolloid carriers, which can be mixed with food. A single administration of AM251 by intravenous injection (at 0.85 or 5mgkg-1BW) or by ingestion of hydrocolloid carriers entrapping AM251 at a concentration of 5mgkg-1BW led to a transient attenuation of food intake. The consequent reduced cumulative food intake and BW were observed in the treated chicks for at least 7h post-administration, with no gender differences. Circulating levels of AM251, assessed by LC-MS following 48h of continuous feeding with hydrocolloid carriers containing 50mg AM251kg-1BWday-1, were physiologically significant at 186±73pmolml-1. It is concluded that unlike some other factors, which act differently in birds compared to mammals such as ghrelin, CB1 inverse agonists attenuate food intake in chicks similar to its effect in mammals. In addition, the new approach for administration of AM251 to birds in hydrocolloid carriers could provide a simple and stress-free tool for prolonged studies of this control mechanism in birds.
AB - The cannabinoid receptor (CB1) was studied primarily in mammals where it was found to comprise a link between reward processes and addictive behavior such as food consumption. The purpose of this study was twofold: first to characterize the effect of the chicken CB1 receptor inverse agonist AM251 on food intake, and second, to establish a stress-free approach for application of AM251 to birds using hydrocolloid carriers, which can be mixed with food. A single administration of AM251 by intravenous injection (at 0.85 or 5mgkg-1BW) or by ingestion of hydrocolloid carriers entrapping AM251 at a concentration of 5mgkg-1BW led to a transient attenuation of food intake. The consequent reduced cumulative food intake and BW were observed in the treated chicks for at least 7h post-administration, with no gender differences. Circulating levels of AM251, assessed by LC-MS following 48h of continuous feeding with hydrocolloid carriers containing 50mg AM251kg-1BWday-1, were physiologically significant at 186±73pmolml-1. It is concluded that unlike some other factors, which act differently in birds compared to mammals such as ghrelin, CB1 inverse agonists attenuate food intake in chicks similar to its effect in mammals. In addition, the new approach for administration of AM251 to birds in hydrocolloid carriers could provide a simple and stress-free tool for prolonged studies of this control mechanism in birds.
KW - AM251
KW - Cannabinoid receptor
KW - Chicken
KW - Hydrocolloid carrier
UR - http://www.scopus.com/inward/record.url?scp=78651424114&partnerID=8YFLogxK
U2 - 10.1016/j.ygcen.2010.11.011
DO - 10.1016/j.ygcen.2010.11.011
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AN - SCOPUS:78651424114
SN - 0016-6480
VL - 170
SP - 522
EP - 527
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 3
ER -