Attenuation of hydrogen peroxide-mediated oxidative stress by Brassica juncea annexin-3 counteracts thiol-specific antioxidant (TSA1) deficiency in Saccharomyces cerevisiae

Ahan Dalal; Abhaypratap Vishwakarma; Naveen Kumar Singh; Triveni Gudla; Mrinal Kanti Bhattacharyya; Kollipara Padmasree; Andrea Viehhauser; Karl Josef Dietz; Pulugurtha Bharadwaja Kirti

doi:10.1016/j.febslet.2014.01.006

Attenuation of hydrogen peroxide-mediated oxidative stress by Brassica juncea annexin-3 counteracts thiol-specific antioxidant (TSA1) deficiency in Saccharomyces cerevisiae

Ahan Dalal^*, Abhaypratap Vishwakarma, Naveen Kumar Singh, Triveni Gudla, Mrinal Kanti Bhattacharyya, Kollipara Padmasree, Andrea Viehhauser, Karl Josef Dietz, Pulugurtha Bharadwaja Kirti

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Brassica juncea annexin-3 (BjAnn3) was functionally characterized for its ability to modulate H₂O₂-mediated oxidative stress in Saccharomyces cerevisiae. BjAnn3 showed a significant protective role in cellular-defense against oxidative stress and partially alleviated inhibition of mitochondrial respiration in presence of exogenously applied H ₂O₂. Heterologous expression of BjAnn3 protected membranes from oxidative stress-mediated damage and positively regulated antioxidant gene expression for ROS detoxification. We conclude that, BjAnn3 partially counteracts the effects of thioredoxin peroxidase 1 (TSA1) deficiency and aids in cellular-protection across kingdoms. Despite partial compensation of TSA1 by BjAnn3 in cell-viability tests, the over-complementation in ROS-related features suggests the existence of both redundant (e.g. ROS detoxification) and distinct features (e.g. membrane protection versus proximity-based redox regulator) of both proteins.

Original language	English
Pages (from-to)	584-593
Number of pages	10
Journal	FEBS Letters
Volume	588
Issue number	4
DOIs	https://doi.org/10.1016/j.febslet.2014.01.006
State	Published - 14 Feb 2014
Externally published	Yes

Bibliographical note

Funding Information:
PBK acknowledges the grant in the form of a research project from the University Grants Commission, India. Facilities used from School of Life Sciences (under DST-FIST and DBT-CREBB), Central Instruments Laboratory and Centre for Nanotechnology at University of Hyderabad, India, are acknowledged. AD gratefully acknowledges UoH-DBT-CREBB, India, and DAAD, Germany, for research fellowships and travel grants. KJD acknowledges support by the German Science Foundation (DFG, Di 346). We thankfully acknowledge Mr. Srikant Prasad Verma, DOEACC society, Kolkata, India, for his contribution of Supplemental Fig. 2 . We thank Ms. M. Nalini for her technical expertise in confocal microscopy.

Keywords

Brassica juncea annexin-3
Complementation
Cross talk
Oxidative stress
Saccharomyces cerevisiae
Thioredoxin peroxidase 1

Access to Document

10.1016/j.febslet.2014.01.006

Cite this

Dalal, A., Vishwakarma, A., Singh, N. K., Gudla, T., Bhattacharyya, M. K., Padmasree, K., Viehhauser, A., Dietz, K. J., & Kirti, P. B. (2014). Attenuation of hydrogen peroxide-mediated oxidative stress by Brassica juncea annexin-3 counteracts thiol-specific antioxidant (TSA1) deficiency in Saccharomyces cerevisiae. FEBS Letters, 588(4), 584-593. https://doi.org/10.1016/j.febslet.2014.01.006

@article{3d988d9058dd4d3a91e19db0f4e9d8c8,

title = "Attenuation of hydrogen peroxide-mediated oxidative stress by Brassica juncea annexin-3 counteracts thiol-specific antioxidant (TSA1) deficiency in Saccharomyces cerevisiae",

abstract = "Brassica juncea annexin-3 (BjAnn3) was functionally characterized for its ability to modulate H2O2-mediated oxidative stress in Saccharomyces cerevisiae. BjAnn3 showed a significant protective role in cellular-defense against oxidative stress and partially alleviated inhibition of mitochondrial respiration in presence of exogenously applied H 2O2. Heterologous expression of BjAnn3 protected membranes from oxidative stress-mediated damage and positively regulated antioxidant gene expression for ROS detoxification. We conclude that, BjAnn3 partially counteracts the effects of thioredoxin peroxidase 1 (TSA1) deficiency and aids in cellular-protection across kingdoms. Despite partial compensation of TSA1 by BjAnn3 in cell-viability tests, the over-complementation in ROS-related features suggests the existence of both redundant (e.g. ROS detoxification) and distinct features (e.g. membrane protection versus proximity-based redox regulator) of both proteins.",

keywords = "Brassica juncea annexin-3, Complementation, Cross talk, Oxidative stress, Saccharomyces cerevisiae, Thioredoxin peroxidase 1",

author = "Ahan Dalal and Abhaypratap Vishwakarma and Singh, {Naveen Kumar} and Triveni Gudla and Bhattacharyya, {Mrinal Kanti} and Kollipara Padmasree and Andrea Viehhauser and Dietz, {Karl Josef} and Kirti, {Pulugurtha Bharadwaja}",

note = "Funding Information: PBK acknowledges the grant in the form of a research project from the University Grants Commission, India. Facilities used from School of Life Sciences (under DST-FIST and DBT-CREBB), Central Instruments Laboratory and Centre for Nanotechnology at University of Hyderabad, India, are acknowledged. AD gratefully acknowledges UoH-DBT-CREBB, India, and DAAD, Germany, for research fellowships and travel grants. KJD acknowledges support by the German Science Foundation (DFG, Di 346). We thankfully acknowledge Mr. Srikant Prasad Verma, DOEACC society, Kolkata, India, for his contribution of Supplemental Fig. 2 . We thank Ms. M. Nalini for her technical expertise in confocal microscopy. ",

year = "2014",

month = feb,

day = "14",

doi = "10.1016/j.febslet.2014.01.006",

language = "אנגלית",

volume = "588",

pages = "584--593",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "4",

}

Dalal, A, Vishwakarma, A, Singh, NK, Gudla, T, Bhattacharyya, MK, Padmasree, K, Viehhauser, A, Dietz, KJ & Kirti, PB 2014, 'Attenuation of hydrogen peroxide-mediated oxidative stress by Brassica juncea annexin-3 counteracts thiol-specific antioxidant (TSA1) deficiency in Saccharomyces cerevisiae', FEBS Letters, vol. 588, no. 4, pp. 584-593. https://doi.org/10.1016/j.febslet.2014.01.006

TY - JOUR

T1 - Attenuation of hydrogen peroxide-mediated oxidative stress by Brassica juncea annexin-3 counteracts thiol-specific antioxidant (TSA1) deficiency in Saccharomyces cerevisiae

AU - Dalal, Ahan

AU - Vishwakarma, Abhaypratap

AU - Singh, Naveen Kumar

AU - Gudla, Triveni

AU - Bhattacharyya, Mrinal Kanti

AU - Padmasree, Kollipara

AU - Viehhauser, Andrea

AU - Dietz, Karl Josef

AU - Kirti, Pulugurtha Bharadwaja

N1 - Funding Information: PBK acknowledges the grant in the form of a research project from the University Grants Commission, India. Facilities used from School of Life Sciences (under DST-FIST and DBT-CREBB), Central Instruments Laboratory and Centre for Nanotechnology at University of Hyderabad, India, are acknowledged. AD gratefully acknowledges UoH-DBT-CREBB, India, and DAAD, Germany, for research fellowships and travel grants. KJD acknowledges support by the German Science Foundation (DFG, Di 346). We thankfully acknowledge Mr. Srikant Prasad Verma, DOEACC society, Kolkata, India, for his contribution of Supplemental Fig. 2 . We thank Ms. M. Nalini for her technical expertise in confocal microscopy.

PY - 2014/2/14

Y1 - 2014/2/14

N2 - Brassica juncea annexin-3 (BjAnn3) was functionally characterized for its ability to modulate H2O2-mediated oxidative stress in Saccharomyces cerevisiae. BjAnn3 showed a significant protective role in cellular-defense against oxidative stress and partially alleviated inhibition of mitochondrial respiration in presence of exogenously applied H 2O2. Heterologous expression of BjAnn3 protected membranes from oxidative stress-mediated damage and positively regulated antioxidant gene expression for ROS detoxification. We conclude that, BjAnn3 partially counteracts the effects of thioredoxin peroxidase 1 (TSA1) deficiency and aids in cellular-protection across kingdoms. Despite partial compensation of TSA1 by BjAnn3 in cell-viability tests, the over-complementation in ROS-related features suggests the existence of both redundant (e.g. ROS detoxification) and distinct features (e.g. membrane protection versus proximity-based redox regulator) of both proteins.

AB - Brassica juncea annexin-3 (BjAnn3) was functionally characterized for its ability to modulate H2O2-mediated oxidative stress in Saccharomyces cerevisiae. BjAnn3 showed a significant protective role in cellular-defense against oxidative stress and partially alleviated inhibition of mitochondrial respiration in presence of exogenously applied H 2O2. Heterologous expression of BjAnn3 protected membranes from oxidative stress-mediated damage and positively regulated antioxidant gene expression for ROS detoxification. We conclude that, BjAnn3 partially counteracts the effects of thioredoxin peroxidase 1 (TSA1) deficiency and aids in cellular-protection across kingdoms. Despite partial compensation of TSA1 by BjAnn3 in cell-viability tests, the over-complementation in ROS-related features suggests the existence of both redundant (e.g. ROS detoxification) and distinct features (e.g. membrane protection versus proximity-based redox regulator) of both proteins.

KW - Brassica juncea annexin-3

KW - Complementation

KW - Cross talk

KW - Oxidative stress

KW - Saccharomyces cerevisiae

KW - Thioredoxin peroxidase 1

UR - http://www.scopus.com/inward/record.url?scp=84896707148&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2014.01.006

DO - 10.1016/j.febslet.2014.01.006

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 24444602

AN - SCOPUS:84896707148

SN - 0014-5793

VL - 588

SP - 584

EP - 593

JO - FEBS Letters

JF - FEBS Letters

IS - 4

ER -

Attenuation of hydrogen peroxide-mediated oxidative stress by Brassica juncea annexin-3 counteracts thiol-specific antioxidant (TSA1) deficiency in Saccharomyces cerevisiae

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this