Attenuation of hydrogen peroxide-mediated oxidative stress by Brassica juncea annexin-3 counteracts thiol-specific antioxidant (TSA1) deficiency in Saccharomyces cerevisiae

Ahan Dalal*, Abhaypratap Vishwakarma, Naveen Kumar Singh, Triveni Gudla, Mrinal Kanti Bhattacharyya, Kollipara Padmasree, Andrea Viehhauser, Karl Josef Dietz, Pulugurtha Bharadwaja Kirti

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Brassica juncea annexin-3 (BjAnn3) was functionally characterized for its ability to modulate H2O2-mediated oxidative stress in Saccharomyces cerevisiae. BjAnn3 showed a significant protective role in cellular-defense against oxidative stress and partially alleviated inhibition of mitochondrial respiration in presence of exogenously applied H 2O2. Heterologous expression of BjAnn3 protected membranes from oxidative stress-mediated damage and positively regulated antioxidant gene expression for ROS detoxification. We conclude that, BjAnn3 partially counteracts the effects of thioredoxin peroxidase 1 (TSA1) deficiency and aids in cellular-protection across kingdoms. Despite partial compensation of TSA1 by BjAnn3 in cell-viability tests, the over-complementation in ROS-related features suggests the existence of both redundant (e.g. ROS detoxification) and distinct features (e.g. membrane protection versus proximity-based redox regulator) of both proteins.

Original languageAmerican English
Pages (from-to)584-593
Number of pages10
JournalFEBS Letters
Volume588
Issue number4
DOIs
StatePublished - 14 Feb 2014
Externally publishedYes

Bibliographical note

Funding Information:
PBK acknowledges the grant in the form of a research project from the University Grants Commission, India. Facilities used from School of Life Sciences (under DST-FIST and DBT-CREBB), Central Instruments Laboratory and Centre for Nanotechnology at University of Hyderabad, India, are acknowledged. AD gratefully acknowledges UoH-DBT-CREBB, India, and DAAD, Germany, for research fellowships and travel grants. KJD acknowledges support by the German Science Foundation (DFG, Di 346). We thankfully acknowledge Mr. Srikant Prasad Verma, DOEACC society, Kolkata, India, for his contribution of Supplemental Fig. 2 . We thank Ms. M. Nalini for her technical expertise in confocal microscopy.

Keywords

  • Brassica juncea annexin-3
  • Complementation
  • Cross talk
  • Oxidative stress
  • Saccharomyces cerevisiae
  • Thioredoxin peroxidase 1

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