Au Nanoparticle-Enhanced Surface Plasmon Resonance Sensing of Biocatalytic Transformations

Maya Zayats, Svetlana P. Pogorelova, Andrei B. Kharitonov, Oleg Lioubashevski, Eugenii Katz, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

N-(3-Aminopropyl)-N′-methyl-4,4′-bipyridinium is coupled to tiopronin-capped Au nanoparticles (diameter ca. 2 nm) to yield methyl(ami-nopropyl)viologen-functionalized Au nanoparticles (MPAV 2+-Au nanoparticles). In situ electrochemical surface plasmon resonance (SPR) measurements are used to follow the electrochemical deposition of the bipyridinium radical cation modified Au nanoparticles on an Au-coated glass surface and the reoxidation and dissolution of the bipyridinium radical cation film. The MPAV2+-functionalized Au nanoparticles are also employed for the amplified SPR detection of NAD+ and NADH cofactors. By SPR monitoring the partial biocatalyzed dissolution of the bipyridinium radical cation film in the presence of diaphorase (DP) NAD+ is detected in the concentration range of 1 × 10-4 M to 2 × 10-3 M. Similarly, the diaphorase-mediated formation of the bipyridinium radical cation film on the Au-coated glass surface by the reduction of the MPAV2+-functionalized Au nanoparticles by NADH is used for the amplified SPR detection of NADH in the concentration range of 1 × 10-4 M to 1 × 10-3 M.

Original languageEnglish
Pages (from-to)6108-6114
Number of pages7
JournalChemistry - A European Journal
Volume9
Issue number24
DOIs
StatePublished - 15 Dec 2003

Keywords

  • Bioelectrocatalysis
  • Biosensors
  • Gold nanoparticles
  • NAD/NADH cofactors
  • Surface plasmon resonance

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