TY - JOUR
T1 - Au Nanoparticle-Enhanced Surface Plasmon Resonance Sensing of Biocatalytic Transformations
AU - Zayats, Maya
AU - Pogorelova, Svetlana P.
AU - Kharitonov, Andrei B.
AU - Lioubashevski, Oleg
AU - Katz, Eugenii
AU - Willner, Itamar
PY - 2003/12/15
Y1 - 2003/12/15
N2 - N-(3-Aminopropyl)-N′-methyl-4,4′-bipyridinium is coupled to tiopronin-capped Au nanoparticles (diameter ca. 2 nm) to yield methyl(ami-nopropyl)viologen-functionalized Au nanoparticles (MPAV 2+-Au nanoparticles). In situ electrochemical surface plasmon resonance (SPR) measurements are used to follow the electrochemical deposition of the bipyridinium radical cation modified Au nanoparticles on an Au-coated glass surface and the reoxidation and dissolution of the bipyridinium radical cation film. The MPAV2+-functionalized Au nanoparticles are also employed for the amplified SPR detection of NAD+ and NADH cofactors. By SPR monitoring the partial biocatalyzed dissolution of the bipyridinium radical cation film in the presence of diaphorase (DP) NAD+ is detected in the concentration range of 1 × 10-4 M to 2 × 10-3 M. Similarly, the diaphorase-mediated formation of the bipyridinium radical cation film on the Au-coated glass surface by the reduction of the MPAV2+-functionalized Au nanoparticles by NADH is used for the amplified SPR detection of NADH in the concentration range of 1 × 10-4 M to 1 × 10-3 M.
AB - N-(3-Aminopropyl)-N′-methyl-4,4′-bipyridinium is coupled to tiopronin-capped Au nanoparticles (diameter ca. 2 nm) to yield methyl(ami-nopropyl)viologen-functionalized Au nanoparticles (MPAV 2+-Au nanoparticles). In situ electrochemical surface plasmon resonance (SPR) measurements are used to follow the electrochemical deposition of the bipyridinium radical cation modified Au nanoparticles on an Au-coated glass surface and the reoxidation and dissolution of the bipyridinium radical cation film. The MPAV2+-functionalized Au nanoparticles are also employed for the amplified SPR detection of NAD+ and NADH cofactors. By SPR monitoring the partial biocatalyzed dissolution of the bipyridinium radical cation film in the presence of diaphorase (DP) NAD+ is detected in the concentration range of 1 × 10-4 M to 2 × 10-3 M. Similarly, the diaphorase-mediated formation of the bipyridinium radical cation film on the Au-coated glass surface by the reduction of the MPAV2+-functionalized Au nanoparticles by NADH is used for the amplified SPR detection of NADH in the concentration range of 1 × 10-4 M to 1 × 10-3 M.
KW - Bioelectrocatalysis
KW - Biosensors
KW - Gold nanoparticles
KW - NAD/NADH cofactors
KW - Surface plasmon resonance
UR - http://www.scopus.com/inward/record.url?scp=0347992923&partnerID=8YFLogxK
U2 - 10.1002/chem.200305104
DO - 10.1002/chem.200305104
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C2 - 14679522
AN - SCOPUS:0347992923
SN - 0947-6539
VL - 9
SP - 6108
EP - 6114
JO - Chemistry - A European Journal
JF - Chemistry - A European Journal
IS - 24
ER -