Abstract
The effect of auranofin on histamine release from immunologic and non-immunologic activated rat peritoneal mast cells cocultured with 3T3 fibroblasts (MC/3T3) was investigated. When MC/3T3 were preincubated with 2 × 10-5 M auranofin and thereafter challenged with anti-IgE antibodies, a maximal inhibition of histamine release (86.2%) was obtained. Non-immunological histamine release induced by compound 48/80, substance P and bradykinin was inhibited to a lesser degree, i.e. 36.0%, 37.6% and 24.0% respectively. Simultaneous incubation of auranofin and the stimulating agents resulted in a higher inhibition of histamine release: anti-IgE antibodies - 92.0%; compound 48/80 - 73.5%; substance P - 46.1%. We conclude that auranofin effectively reduces histamine release from immunologic and non-immunologic activated mast cells. This may be relevant to the control of allergic reactions.
| Original language | English |
|---|---|
| Pages (from-to) | 403-407 |
| Number of pages | 5 |
| Journal | International Journal of Immunopharmacology |
| Volume | 12 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1990 |
Bibliographical note
Funding Information:Acknowledgements -- This work was supported by a joint grant of the Hebrew University Hadassah Medical School. We wish to thank Mrs Ana Fibach for her skilful secretarial help.