Auranofin inhibits histamine release from rat peritoneal mast cells

Meir Shalit; Francesca Levi-Schaffer

doi:10.1016/0192-0561(90)90022-F

Auranofin inhibits histamine release from rat peritoneal mast cells

Meir Shalit
, Francesca Levi-Schaffer^*

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The effect of auranofin on histamine release from immunologic and non-immunologic activated rat peritoneal mast cells cocultured with 3T3 fibroblasts (MC/3T3) was investigated. When MC/3T3 were preincubated with 2 × 10^-5 M auranofin and thereafter challenged with anti-IgE antibodies, a maximal inhibition of histamine release (86.2%) was obtained. Non-immunological histamine release induced by compound 48/80, substance P and bradykinin was inhibited to a lesser degree, i.e. 36.0%, 37.6% and 24.0% respectively. Simultaneous incubation of auranofin and the stimulating agents resulted in a higher inhibition of histamine release: anti-IgE antibodies - 92.0%; compound 48/80 - 73.5%; substance P - 46.1%. We conclude that auranofin effectively reduces histamine release from immunologic and non-immunologic activated mast cells. This may be relevant to the control of allergic reactions.

Original language	English
Pages (from-to)	403-407
Number of pages	5
Journal	International Journal of Immunopharmacology
Volume	12
Issue number	4
DOIs	https://doi.org/10.1016/0192-0561(90)90022-F
State	Published - 1990

Bibliographical note

Funding Information:
Acknowledgements -- This work was supported by a joint grant of the Hebrew University Hadassah Medical School. We wish to thank Mrs Ana Fibach for her skilful secretarial help.

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10.1016/0192-0561(90)90022-F

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@article{bcc74402b4184141b0a5d3650376bf01,

title = "Auranofin inhibits histamine release from rat peritoneal mast cells",

abstract = "The effect of auranofin on histamine release from immunologic and non-immunologic activated rat peritoneal mast cells cocultured with 3T3 fibroblasts (MC/3T3) was investigated. When MC/3T3 were preincubated with 2 × 10-5 M auranofin and thereafter challenged with anti-IgE antibodies, a maximal inhibition of histamine release (86.2\%) was obtained. Non-immunological histamine release induced by compound 48/80, substance P and bradykinin was inhibited to a lesser degree, i.e. 36.0\%, 37.6\% and 24.0\% respectively. Simultaneous incubation of auranofin and the stimulating agents resulted in a higher inhibition of histamine release: anti-IgE antibodies - 92.0\%; compound 48/80 - 73.5\%; substance P - 46.1\%. We conclude that auranofin effectively reduces histamine release from immunologic and non-immunologic activated mast cells. This may be relevant to the control of allergic reactions.",

author = "Meir Shalit and Francesca Levi-Schaffer",

note = "Funding Information: Acknowledgements -- This work was supported by a joint grant of the Hebrew University Hadassah Medical School. We wish to thank Mrs Ana Fibach for her skilful secretarial help.",

year = "1990",

doi = "10.1016/0192-0561(90)90022-F",

language = "אנגלית",

volume = "12",

pages = "403--407",

journal = "International Journal of Immunopharmacology",

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T1 - Auranofin inhibits histamine release from rat peritoneal mast cells

AU - Shalit, Meir

AU - Levi-Schaffer, Francesca

N1 - Funding Information: Acknowledgements -- This work was supported by a joint grant of the Hebrew University Hadassah Medical School. We wish to thank Mrs Ana Fibach for her skilful secretarial help.

PY - 1990

Y1 - 1990

N2 - The effect of auranofin on histamine release from immunologic and non-immunologic activated rat peritoneal mast cells cocultured with 3T3 fibroblasts (MC/3T3) was investigated. When MC/3T3 were preincubated with 2 × 10-5 M auranofin and thereafter challenged with anti-IgE antibodies, a maximal inhibition of histamine release (86.2%) was obtained. Non-immunological histamine release induced by compound 48/80, substance P and bradykinin was inhibited to a lesser degree, i.e. 36.0%, 37.6% and 24.0% respectively. Simultaneous incubation of auranofin and the stimulating agents resulted in a higher inhibition of histamine release: anti-IgE antibodies - 92.0%; compound 48/80 - 73.5%; substance P - 46.1%. We conclude that auranofin effectively reduces histamine release from immunologic and non-immunologic activated mast cells. This may be relevant to the control of allergic reactions.

AB - The effect of auranofin on histamine release from immunologic and non-immunologic activated rat peritoneal mast cells cocultured with 3T3 fibroblasts (MC/3T3) was investigated. When MC/3T3 were preincubated with 2 × 10-5 M auranofin and thereafter challenged with anti-IgE antibodies, a maximal inhibition of histamine release (86.2%) was obtained. Non-immunological histamine release induced by compound 48/80, substance P and bradykinin was inhibited to a lesser degree, i.e. 36.0%, 37.6% and 24.0% respectively. Simultaneous incubation of auranofin and the stimulating agents resulted in a higher inhibition of histamine release: anti-IgE antibodies - 92.0%; compound 48/80 - 73.5%; substance P - 46.1%. We conclude that auranofin effectively reduces histamine release from immunologic and non-immunologic activated mast cells. This may be relevant to the control of allergic reactions.

UR - https://www.scopus.com/pages/publications/0025376401

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DO - 10.1016/0192-0561(90)90022-F

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C2 - 1697286

AN - SCOPUS:0025376401

SN - 0192-0561

VL - 12

SP - 403

EP - 407

JO - International Journal of Immunopharmacology

JF - International Journal of Immunopharmacology

IS - 4

ER -

Auranofin inhibits histamine release from rat peritoneal mast cells

Abstract

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