Abstract
Chloroplast fragments suspended in buffer form chlrophyllide. Chlorophyllide formation is enhanced by 0.2-1.0% (v/v) of the non-ionic detergent Triton X-100 and the cationic detergent cetyltrimethylammonium bromide (CTMB), but not by the anionic detergent sodium dodecyl sulfate (SDS). Chloroplast fragments from tissues with high chlorophyllase (chlorophyll-chlorophyllido hydrolase, EC 3.1.1.14) activity (e.g. Citrus leaves) form more chlorophyllide than those from tissues with low chlorophyllase activity (e.g. parsley leaves). Data concerning pH optimum, temperature effects and cofactor requirements of the reaction are presented. The 'autolysis' of chlorophyll appears to be brought about by enzymatic activity of chlorophyllase which upon membrane disruption and solubilization obtains access to its chlorophyll substrate.
Original language | English |
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Pages (from-to) | 201-206 |
Number of pages | 6 |
Journal | Plant Science |
Volume | 43 |
Issue number | 3 |
DOIs | |
State | Published - 1986 |
Keywords
- chlorophyll-degradation
- chlorophyllase
- chlorophyllide