Automated glycan assembly using the glyconeer 2.1 synthesizer

Heung Sik Hahm, Mark K. Schlegel, Mattan Hurevich, Steffen Eller, Frank Schuhmacher, Johanna Hofmann, Kevin Pagel, Peter H. Seeberger*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

Reliable and rapid access to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological research and medical practice. Similarly, the procurement of defined glycans is key to establishing structure-activity relationships and thereby progress in the glycosciences. Here, we describe the rapid assembly of oligosaccharides using the commercially available Glyconeer 2.1 automated glycan synthesizer, monosaccharide building blocks, and a linker-functionalized polystyrene solid support. Purification and quality-control protocols for the oligosaccharide products have been standardized. Synthetic glycans prepared in this way are useful reagents as the basis for glycan arrays, diagnostics, and carbohydratebased vaccines.

Original languageAmerican English
Pages (from-to)E3385-E3389
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number17
DOIs
StatePublished - 25 Apr 2017

Bibliographical note

Funding Information:
We are grateful to the Max Planck Society and the European Research Council (ERC Advanced Grant AUTOHEPARIN to P.H.S.) for generous financial support. M.H. thanks the Max Planck Society for the Minerva Postdoctoral Fellowship. J.H. and K.P. thank the Free University Berlin for generous financial support.

Keywords

  • Automated synthesis
  • Glycobiology
  • Glycochemistry
  • Glyconeer
  • Oligosaccharide synthesis

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