Automated glycan assembly using the glyconeer 2.1 synthesizer

Heung Sik Hahm; Mark K. Schlegel; Mattan Hurevich; Steffen Eller; Frank Schuhmacher; Johanna Hofmann; Kevin Pagel; Peter H. Seeberger

doi:10.1073/pnas.1700141114

Automated glycan assembly using the glyconeer 2.1 synthesizer

Heung Sik Hahm, Mark K. Schlegel, Mattan Hurevich, Steffen Eller, Frank Schuhmacher, Johanna Hofmann, Kevin Pagel, Peter H. Seeberger^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

96 Scopus citations

Abstract

Reliable and rapid access to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological research and medical practice. Similarly, the procurement of defined glycans is key to establishing structure-activity relationships and thereby progress in the glycosciences. Here, we describe the rapid assembly of oligosaccharides using the commercially available Glyconeer 2.1 automated glycan synthesizer, monosaccharide building blocks, and a linker-functionalized polystyrene solid support. Purification and quality-control protocols for the oligosaccharide products have been standardized. Synthetic glycans prepared in this way are useful reagents as the basis for glycan arrays, diagnostics, and carbohydratebased vaccines.

Original language	English
Pages (from-to)	E3385-E3389
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	114
Issue number	17
DOIs	https://doi.org/10.1073/pnas.1700141114
State	Published - 25 Apr 2017

Bibliographical note

Funding Information:
We are grateful to the Max Planck Society and the European Research Council (ERC Advanced Grant AUTOHEPARIN to P.H.S.) for generous financial support. M.H. thanks the Max Planck Society for the Minerva Postdoctoral Fellowship. J.H. and K.P. thank the Free University Berlin for generous financial support.

Keywords

Automated synthesis
Glycobiology
Glycochemistry
Glyconeer
Oligosaccharide synthesis

Access to Document

10.1073/pnas.1700141114

Cite this

@article{96e20c28df5e4e6c8f22c833ea3a137c,

title = "Automated glycan assembly using the glyconeer 2.1 synthesizer",

abstract = "Reliable and rapid access to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological research and medical practice. Similarly, the procurement of defined glycans is key to establishing structure-activity relationships and thereby progress in the glycosciences. Here, we describe the rapid assembly of oligosaccharides using the commercially available Glyconeer 2.1 automated glycan synthesizer, monosaccharide building blocks, and a linker-functionalized polystyrene solid support. Purification and quality-control protocols for the oligosaccharide products have been standardized. Synthetic glycans prepared in this way are useful reagents as the basis for glycan arrays, diagnostics, and carbohydratebased vaccines.",

keywords = "Automated synthesis, Glycobiology, Glycochemistry, Glyconeer, Oligosaccharide synthesis",

author = "Hahm, {Heung Sik} and Schlegel, {Mark K.} and Mattan Hurevich and Steffen Eller and Frank Schuhmacher and Johanna Hofmann and Kevin Pagel and Seeberger, {Peter H.}",

note = "Funding Information: We are grateful to the Max Planck Society and the European Research Council (ERC Advanced Grant AUTOHEPARIN to P.H.S.) for generous financial support. M.H. thanks the Max Planck Society for the Minerva Postdoctoral Fellowship. J.H. and K.P. thank the Free University Berlin for generous financial support.",

year = "2017",

month = apr,

day = "25",

doi = "10.1073/pnas.1700141114",

language = "אנגלית",

volume = "114",

pages = "E3385--E3389",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "17",

}

TY - JOUR

T1 - Automated glycan assembly using the glyconeer 2.1 synthesizer

AU - Hahm, Heung Sik

AU - Schlegel, Mark K.

AU - Hurevich, Mattan

AU - Eller, Steffen

AU - Schuhmacher, Frank

AU - Hofmann, Johanna

AU - Pagel, Kevin

AU - Seeberger, Peter H.

N1 - Funding Information: We are grateful to the Max Planck Society and the European Research Council (ERC Advanced Grant AUTOHEPARIN to P.H.S.) for generous financial support. M.H. thanks the Max Planck Society for the Minerva Postdoctoral Fellowship. J.H. and K.P. thank the Free University Berlin for generous financial support.

PY - 2017/4/25

Y1 - 2017/4/25

N2 - Reliable and rapid access to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological research and medical practice. Similarly, the procurement of defined glycans is key to establishing structure-activity relationships and thereby progress in the glycosciences. Here, we describe the rapid assembly of oligosaccharides using the commercially available Glyconeer 2.1 automated glycan synthesizer, monosaccharide building blocks, and a linker-functionalized polystyrene solid support. Purification and quality-control protocols for the oligosaccharide products have been standardized. Synthetic glycans prepared in this way are useful reagents as the basis for glycan arrays, diagnostics, and carbohydratebased vaccines.

AB - Reliable and rapid access to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological research and medical practice. Similarly, the procurement of defined glycans is key to establishing structure-activity relationships and thereby progress in the glycosciences. Here, we describe the rapid assembly of oligosaccharides using the commercially available Glyconeer 2.1 automated glycan synthesizer, monosaccharide building blocks, and a linker-functionalized polystyrene solid support. Purification and quality-control protocols for the oligosaccharide products have been standardized. Synthetic glycans prepared in this way are useful reagents as the basis for glycan arrays, diagnostics, and carbohydratebased vaccines.

KW - Automated synthesis

KW - Glycobiology

KW - Glycochemistry

KW - Glyconeer

KW - Oligosaccharide synthesis

UR - http://www.scopus.com/inward/record.url?scp=85018809643&partnerID=8YFLogxK

U2 - 10.1073/pnas.1700141114

DO - 10.1073/pnas.1700141114

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 28396442

AN - SCOPUS:85018809643

SN - 0027-8424

VL - 114

SP - E3385-E3389

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 17

ER -

Automated glycan assembly using the glyconeer 2.1 synthesizer

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this