Autonomous fueled mechanical replication of nucleic acid templates for the amplified optical detection of DNA

Yossi Weizmann; Zoya Cheglakov; Valeri Pavlov; Itamar Willner

doi:10.1002/anie.200503810

Autonomous fueled mechanical replication of nucleic acid templates for the amplified optical detection of DNA

Yossi Weizmann^*, Zoya Cheglakov, Valeri Pavlov, Itamar Willner

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

(Figure Presented) Fuel for a DNA machine: A Fokl enzyme/DNA scission machine is triggered by the hybridization of its DNA hairpin structure with a target nucleic acid. The machine is fueled by a secondary fluorophore (F)- and quencher (Q)-functionalized nucleic acid 2. Scission of 3 leads to replication of the machinery cutter 1 and yields a fluorescent product 6, which provides an optical output for the machine (see scheme).

Original language	English
Pages (from-to)	2238-2242
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	45
Issue number	14
DOIs	https://doi.org/10.1002/anie.200503810
State	Published - 27 Mar 2006

Keywords

DNA
Enzymes
Fluorescence
Self-replication

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10.1002/anie.200503810

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abstract = "(Figure Presented) Fuel for a DNA machine: A Fokl enzyme/DNA scission machine is triggered by the hybridization of its DNA hairpin structure with a target nucleic acid. The machine is fueled by a secondary fluorophore (F)- and quencher (Q)-functionalized nucleic acid 2. Scission of 3 leads to replication of the machinery cutter 1 and yields a fluorescent product 6, which provides an optical output for the machine (see scheme).",

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AU - Cheglakov, Zoya

AU - Pavlov, Valeri

AU - Willner, Itamar

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Y1 - 2006/3/27

N2 - (Figure Presented) Fuel for a DNA machine: A Fokl enzyme/DNA scission machine is triggered by the hybridization of its DNA hairpin structure with a target nucleic acid. The machine is fueled by a secondary fluorophore (F)- and quencher (Q)-functionalized nucleic acid 2. Scission of 3 leads to replication of the machinery cutter 1 and yields a fluorescent product 6, which provides an optical output for the machine (see scheme).

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KW - DNA

KW - Enzymes

KW - Fluorescence

KW - Self-replication

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Autonomous fueled mechanical replication of nucleic acid templates for the amplified optical detection of DNA

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Keywords

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