Autophagy deficiency compromises alternative pathways of respiration following energy deprivation in arabidopsis thaliana

Jessica A.S. Barros, João Henrique F. Cavalcanti, David B. Medeiros, Adriano Nunes-Nesi, Tamar Avin-Wittenberg, Alisdair R. Fernie, Wagner L. Araújo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

Under heterotrophic conditions, carbohydrate oxidation inside the mitochondrion is the primary energy source for cellular metabolism. However, during energy-limited conditions, alternative substrates are required to support respiration. Amino acid oxidation in plant cells plays a key role in this by generating electrons that can be transferred to the mitochondrial electron transport chain via the electron transfer flavoprotein/ubiquinone oxidoreductase system. Autophagy, a catabolic mechanism for macromolecule and protein recycling, allows the maintenance of amino acid pools and nutrient remobilization. Although the association between autophagy and alternative respiratory substrates has been suggested, the extent to which autophagy and primary metabolism interact to support plant respiration remains unclear. To investigate the metabolic importance of autophagy during development and under extended darkness, Arabidopsis (Arabidopsis thaliana) mutants with disruption of autophagy (atg mutants) were used. Under normal growth conditions, atg mutants showed lower growth and seed production with no impact on photosynthesis. Following extended darkness, atg mutants were characterized by signatures of early senescence, including decreased chlorophyll content and maximum photochemical efficiency of photosystem II coupled with increases in dark respiration. Transcript levels of genes involved in alternative pathways of respiration and amino acid catabolism were up-regulated in atg mutants. The metabolite profiles of dark-treated leaves revealed an extensive metabolic reprogramming in which increases in amino acid levels were partially compromised in atg mutants. Although an enhanced respiration in atg mutants was observed during extended darkness, autophagy deficiency compromises protein degradation and the generation of amino acids used as alternative substrates to the respiration.

Original languageAmerican English
Pages (from-to)62-76
Number of pages15
JournalPlant Physiology
Volume175
Issue number1
DOIs
StatePublished - Sep 2017

Bibliographical note

Publisher Copyright:
© 2017 American Society of Plant Biologists. All rights reserved.

Fingerprint

Dive into the research topics of 'Autophagy deficiency compromises alternative pathways of respiration following energy deprivation in arabidopsis thaliana'. Together they form a unique fingerprint.

Cite this