Autorepression of yeast Hsp70 cochaperones by intramolecular interactions involving their J-domains

Mathieu E. Rebeaud, Satyam Tiwari, Bruno Fauvet, Adelaïde Mohr, Pierre Goloubinoff*, Paolo De Los Rios

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The 70 kDa heat shock protein (Hsp70) chaperones control protein homeostasis in all ATP-containing cellular compartments. J-domain proteins (JDPs) coevolved with Hsp70s to trigger ATP hydrolysis and catalytically upload various substrate polypeptides in need to be structurally modified by the chaperone. Here, we measured the protein disaggregation and refolding activities of the main yeast cytosolic Hsp70, Ssa1, in the presence of its most abundant JDPs, Sis1 and Ydj1, and two swap mutants, in which the J-domains have been interchanged. The observed differences by which the four constructs differently cooperate with Ssa1 and cooperate with each other, as well as their observed intrinsic ability to bind misfolded substrates and trigger Ssa1′s ATPase, indicate the presence of yet uncharacterized intramolecular dynamic interactions between the J-domains and the remaining C-terminal segments of these proteins. Taken together, the data suggest an autoregulatory role to these intramolecular interactions within both type A and B JDPs, which might have evolved to reduce energy-costly ATPase cycles by the Ssa1–4 chaperones that are the most abundant Hsp70s in the yeast cytosol.

Original languageEnglish
Pages (from-to)338-348
Number of pages11
JournalCell Stress and Chaperones
Volume29
Issue number2
DOIs
StatePublished - Apr 2024
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2024 The Authors

Keywords

  • Autorepression
  • Coevolution
  • DNAJA
  • DNAJB
  • JDPs

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