A sensitive assay for glycerol and other polyols was developed, based on periodate oxidation to formaldehyde, followed by a colorimetric assay with 3-methyl-2-benzothiazolone hydrazone. Apparent glycerol concentrations thus measured in saltern crystallizer ponds were around 20-36 μM, while in the Dead Sea, during a Dunaliella bloom, values were up to 27 μM. However, these values probably overestimate the glycerol concentrations present, as shown by labeled glycerol uptake experiments. Values of [K + Sn] (natural concentration + affinity constant) in saltern ponds were as low as 0.76-1.4 μM, with Vmax values of 193-303 nmol 1-1h-1, and turnover times between 2.6-7.2 h at 35°C. Similar measurements in the Dead Sea were: [K + Sn] 0.07-1.41 μM, Vmax values 160-426 nmol 1-1h-1, and turnover times in the range of 0.45-3.3 h.
|Original language||American English|
|Number of pages||9|
|Journal||FEMS Microbiology Ecology|
|State||Published - May 1993|
- Dead Sea