TY - JOUR
T1 - Availability, uptake and turnover of glycerol in hypersaline environments
AU - Oren, Aharon
PY - 1993/5
Y1 - 1993/5
N2 - A sensitive assay for glycerol and other polyols was developed, based on periodate oxidation to formaldehyde, followed by a colorimetric assay with 3-methyl-2-benzothiazolone hydrazone. Apparent glycerol concentrations thus measured in saltern crystallizer ponds were around 20-36 μM, while in the Dead Sea, during a Dunaliella bloom, values were up to 27 μM. However, these values probably overestimate the glycerol concentrations present, as shown by labeled glycerol uptake experiments. Values of [K + Sn] (natural concentration + affinity constant) in saltern ponds were as low as 0.76-1.4 μM, with Vmax values of 193-303 nmol 1-1h-1, and turnover times between 2.6-7.2 h at 35°C. Similar measurements in the Dead Sea were: [K + Sn] 0.07-1.41 μM, Vmax values 160-426 nmol 1-1h-1, and turnover times in the range of 0.45-3.3 h.
AB - A sensitive assay for glycerol and other polyols was developed, based on periodate oxidation to formaldehyde, followed by a colorimetric assay with 3-methyl-2-benzothiazolone hydrazone. Apparent glycerol concentrations thus measured in saltern crystallizer ponds were around 20-36 μM, while in the Dead Sea, during a Dunaliella bloom, values were up to 27 μM. However, these values probably overestimate the glycerol concentrations present, as shown by labeled glycerol uptake experiments. Values of [K + Sn] (natural concentration + affinity constant) in saltern ponds were as low as 0.76-1.4 μM, with Vmax values of 193-303 nmol 1-1h-1, and turnover times between 2.6-7.2 h at 35°C. Similar measurements in the Dead Sea were: [K + Sn] 0.07-1.41 μM, Vmax values 160-426 nmol 1-1h-1, and turnover times in the range of 0.45-3.3 h.
KW - Dead Sea
KW - Glycerol
KW - Halobacterium
KW - Salterns
UR - http://www.scopus.com/inward/record.url?scp=0027336457&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6941.1993.tb00012.x
DO - 10.1111/j.1574-6941.1993.tb00012.x
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AN - SCOPUS:0027336457
SN - 0168-6496
VL - 12
SP - 15
EP - 23
JO - FEMS Microbiology Ecology
JF - FEMS Microbiology Ecology
IS - 1
ER -