TY - JOUR
T1 - B-lymphocytes in CLL and NHL differ in the mRNA splicing pattern of the CD45 molecule
AU - Yu, Y.
AU - Rabinowitz, R.
AU - Polliack, A.
AU - Ben-Bassat, H.
AU - Schlesinger, Michael
PY - 2000
Y1 - 2000
N2 - In the present study the cell surface expression of CD45 isoforms on normal and neoplastic human B cells was correlated with splice products of the CD45 mRNA, using RT-PCR technology. In non-Hodgkin's lymphoma cells in the leukemic phase (NHL) the majority of the cells expressed a high level of CD45RA, while in CLL most of the cells expressed a low level. In the Raji and Daudi Burkitt B-cell lymphoma lines the main CD45 mRNA product was the largest, unspliced, full-length isoform (456) and the 56 splice product. Similar results were obtained with B-cell lymphoma cells isolated from the peripheral blood of patients with NHL in the leukemic phase. In EBV- transformed B-cell lines, the 456 and the 56 isoform of CD45 mRNA were predominant, but in addition a low level of the 5- and 0-exon splice products was detected. A strikingly different pattern was obtained with B-CLL cells. In CLL the level of the 456 and the 56 isoforms was low, while that of the 5- and 0-exon splice products was increased. Thus, in contrast to the heterogeneity in the expression of CD45RO in B-CLL, the majority of the cells contained the CD45 mRNA splice product coding for CD45RO. Analysis of splice products of the CD45 mRNA may serve as an additional tool to differentiate CLL from the leukemic phase of NHL. (C) Munksgaard 2000.
AB - In the present study the cell surface expression of CD45 isoforms on normal and neoplastic human B cells was correlated with splice products of the CD45 mRNA, using RT-PCR technology. In non-Hodgkin's lymphoma cells in the leukemic phase (NHL) the majority of the cells expressed a high level of CD45RA, while in CLL most of the cells expressed a low level. In the Raji and Daudi Burkitt B-cell lymphoma lines the main CD45 mRNA product was the largest, unspliced, full-length isoform (456) and the 56 splice product. Similar results were obtained with B-cell lymphoma cells isolated from the peripheral blood of patients with NHL in the leukemic phase. In EBV- transformed B-cell lines, the 456 and the 56 isoform of CD45 mRNA were predominant, but in addition a low level of the 5- and 0-exon splice products was detected. A strikingly different pattern was obtained with B-CLL cells. In CLL the level of the 456 and the 56 isoforms was low, while that of the 5- and 0-exon splice products was increased. Thus, in contrast to the heterogeneity in the expression of CD45RO in B-CLL, the majority of the cells contained the CD45 mRNA splice product coding for CD45RO. Analysis of splice products of the CD45 mRNA may serve as an additional tool to differentiate CLL from the leukemic phase of NHL. (C) Munksgaard 2000.
KW - CD45 isoforms
KW - CD45 mRNA
KW - CLL
KW - NHL
UR - http://www.scopus.com/inward/record.url?scp=0033934947&partnerID=8YFLogxK
U2 - 10.1034/j.1600-0609.2000.90144.x
DO - 10.1034/j.1600-0609.2000.90144.x
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 10901591
AN - SCOPUS:0033934947
SN - 0902-4441
VL - 64
SP - 376
EP - 384
JO - European Journal of Haematology
JF - European Journal of Haematology
IS - 6
ER -