TY - JOUR
T1 - Babesiosis caused by Babesia vogeli in dogs from Uberlândia State of Minas Gerais, Brazil
AU - Barbosa, Camila Oliveira Silva
AU - Garcia, Jaqueline Rosa
AU - de Melo Nasser Fava, Natália
AU - Pereira, Douglas Alves
AU - da Cunha, Maria Júlia Rodrigues
AU - Nachum-Biala, Yaarit
AU - Cury, Márcia Cristina
AU - Baneth, Gad
N1 - Publisher Copyright:
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Babesia is tick-transmitted protozoan parasites that infect mammalian hosts and have a major impact on farm and pet health-associated costs worldwide. This study aimed to test the prevalence of Babesia spp. infection in a small cohort of dogs at a veterinary hospital and to perform molecular characterization of the Babesia species causing the infection. For the PCR assay, 5 mL of blood was collected by venipuncture of the cephalic or radial veins in 300 dogs of different ages, sex, and breeds, which were presented to the veterinary hospital of the Federal University of Uberlândia between March 2015 and April 2016. In addition, a drop of blood was collected from the marginal blood vessels of the ear of dogs included in this study. Ninety-two (30.67%) were positive for Babesia spp., as determined by microscopic observation of the blood smear, revealing the presence of intra-erythrocyte merozoites. For molecular characterization by PCR, 17 samples were chosen from dogs who were tested positive for Babesia spp. by blood smears. Among them, B. vogeli was found to infect all 17 dogs, as determined by 99–100% sequence identity (closest GenBank match KT246307) using primers PIRO A/PIRO B. Our results indicate that the species observed in these dogs was B. vogeli.
AB - Babesia is tick-transmitted protozoan parasites that infect mammalian hosts and have a major impact on farm and pet health-associated costs worldwide. This study aimed to test the prevalence of Babesia spp. infection in a small cohort of dogs at a veterinary hospital and to perform molecular characterization of the Babesia species causing the infection. For the PCR assay, 5 mL of blood was collected by venipuncture of the cephalic or radial veins in 300 dogs of different ages, sex, and breeds, which were presented to the veterinary hospital of the Federal University of Uberlândia between March 2015 and April 2016. In addition, a drop of blood was collected from the marginal blood vessels of the ear of dogs included in this study. Ninety-two (30.67%) were positive for Babesia spp., as determined by microscopic observation of the blood smear, revealing the presence of intra-erythrocyte merozoites. For molecular characterization by PCR, 17 samples were chosen from dogs who were tested positive for Babesia spp. by blood smears. Among them, B. vogeli was found to infect all 17 dogs, as determined by 99–100% sequence identity (closest GenBank match KT246307) using primers PIRO A/PIRO B. Our results indicate that the species observed in these dogs was B. vogeli.
KW - Blood smear
KW - Canine diseases
KW - Hemoparasites
KW - PCR
KW - Tick borne
UR - http://www.scopus.com/inward/record.url?scp=85081638189&partnerID=8YFLogxK
U2 - 10.1007/s00436-019-06515-3
DO - 10.1007/s00436-019-06515-3
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C2 - 32140779
AN - SCOPUS:85081638189
SN - 0932-0113
VL - 119
SP - 1173
EP - 1176
JO - Parasitology Research
JF - Parasitology Research
IS - 3
ER -