TY - JOUR
T1 - Bacterial genotoxicity bioreporters
AU - Biran, Alva
AU - Yagur-Kroll, Sharon
AU - Pedahzur, Rami
AU - Buchinger, Sebastian
AU - Reifferscheid, Georg
AU - Ben-Yoav, Hadar
AU - Shacham-Diamand, Yosi
AU - Belkin, Shimshon
PY - 2010/7
Y1 - 2010/7
N2 - Ever since the introduction of the Salmonella typhimurium mammalian microsome mutagenicity assay (the 'Ames test') over three decades ago, there has been a constant development of additional genotoxicity assays based upon the use of genetically engineered microorganisms. Such assays rely either on reversion principles similar to those of the Ames test, or on promoter-reporter fusions that generate a quantifiable dose-dependent signal in the presence of potential DNA damaging compounds and the induction of repair mechanisms; the latter group is the subject of the present review. Some of these assays were only briefly described in the scientific literature, whereas others have been developed all the way to commercial products. Out of these, only one, the umu-test, has been fully validated and ISO- and OECD standardized. Here we review the main directions undertaken in the construction and testing of bacterial-based genotoxicity bioassays, including the attempts to incorporate at least a partial metabolic activation capacity into the molecular design. We list the genetic modifications introduced into the tester strains, compare the performance of the different assays, and briefly describe the first attempts to incorporate such bacterial reporters into actual genotoxicity testing devices.
AB - Ever since the introduction of the Salmonella typhimurium mammalian microsome mutagenicity assay (the 'Ames test') over three decades ago, there has been a constant development of additional genotoxicity assays based upon the use of genetically engineered microorganisms. Such assays rely either on reversion principles similar to those of the Ames test, or on promoter-reporter fusions that generate a quantifiable dose-dependent signal in the presence of potential DNA damaging compounds and the induction of repair mechanisms; the latter group is the subject of the present review. Some of these assays were only briefly described in the scientific literature, whereas others have been developed all the way to commercial products. Out of these, only one, the umu-test, has been fully validated and ISO- and OECD standardized. Here we review the main directions undertaken in the construction and testing of bacterial-based genotoxicity bioassays, including the attempts to incorporate at least a partial metabolic activation capacity into the molecular design. We list the genetic modifications introduced into the tester strains, compare the performance of the different assays, and briefly describe the first attempts to incorporate such bacterial reporters into actual genotoxicity testing devices.
UR - http://www.scopus.com/inward/record.url?scp=77953556161&partnerID=8YFLogxK
U2 - 10.1111/j.1751-7915.2009.00160.x
DO - 10.1111/j.1751-7915.2009.00160.x
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C2 - 21255340
AN - SCOPUS:77953556161
SN - 1751-7907
VL - 3
SP - 412
EP - 427
JO - Microbial Biotechnology
JF - Microbial Biotechnology
IS - 4
ER -