Bacterial membrane potential analyzed by spectrofluorocytometry

Murray Resnick, Shimon Schuldiner, Herve Bercovier*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Cells of Mycobacterium smegmatis 607 in their late exponential growth were stained with rhodamine 123. The stained bacterial populations were analyzed by fluorocytometry and spectrofluorometry. Responses of the bacterial membrane potential to agents such as valinomycin, graimicidin, nigericin, and sodium azide were analyzed with the two techniques mentioned above. The results have shown that the flow cytometric technique allowed detection of changes in bacterial membrane potential and allowed population response analysis to ionophore activity. The applications of this technique are promising for the rapid detection of membrane potential mutants and for the estimation of the viability of a bacterial population.

Original languageEnglish
Pages (from-to)183-185
Number of pages3
JournalCurrent Microbiology
Volume12
Issue number4
DOIs
StatePublished - Jul 1985

Fingerprint

Dive into the research topics of 'Bacterial membrane potential analyzed by spectrofluorocytometry'. Together they form a unique fingerprint.

Cite this