Bacteriophage rescue therapy of a vancomycin-resistant enterococcus faecium infection in a one-year-old child following a third liver transplantation

Kevin Paul*, Maya Merabishvili, Ronen Hazan*, Martin Christner, Uta Herden, Daniel Gelman, Leron Khalifa, Ortal Yerushalmy, Shunit Coppenhagen-Glazer, Theresa Harbauer, Sebastian Schulz-Jürgensen, Holger Rohde, Lutz Fischer, Saima Aslam, Christine Rohde, Ran Nir-Paz, Jean Paul Pirnay*, Dominique Singer, Ania Carolina Muntau

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Phage therapy is an experimental therapeutic approach used to target multidrug-resistant bacterial infections. A lack of reliable data with regard to its efficacy and regulatory hurdles hinders a broad application. Here we report, for the first time, a case of vancomycin-resistant Enterococcus faecium abdominal infection in a one-year-old, critically ill, and three times liver transplanted girl, which was successfully treated with intravenous injections (twice per day for 20 days) of a magistral preparation containing two Enterococcus phages. This correlated with a reduction in baseline C-reactive protein (CRP), successful weaning from mechanical ventilation and without associated clinical adverse events. Prior to clinical use, phage genome was sequenced to confirm the absence of genetic determinants conferring lysogeny, virulence or antibiotic resistance, and thus their safety. Using a phage neutralization assay, no neutralizing anti-phage antibodies in the patient’s serum could be detected. Vancomycin-susceptible E. faecium isolates were identified in close relation to phage therapy and, by using whole-genome sequencing, it was demonstrated that vancomycin-susceptible E. faecium emerged from vancomycin-resistant progenitors. Covering a one year follow up, we provide further evidence for the feasibility of bacteriophage therapy that can serve as a basis for urgently needed controlled clinical trials.

Original languageAmerican English
Article number1785
JournalViruses
Volume13
Issue number9
DOIs
StatePublished - 7 Sep 2021

Bibliographical note

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Bacteriophage
  • Biliary atresia
  • Critical care
  • Enterococcus faecium
  • Liver transplantation
  • Multi-drug resistance
  • Pediatric
  • Vancomycin

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