Bacterioruberin and salinixanthin carotenoids of extremely halophilic Archaea and Bacteria: A Raman spectroscopic study

J. Jehlička*, H. G.M. Edwards, A. Oren

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

79 Scopus citations

Abstract

Laboratory cultures of a number of red extremely halophilic Archaea (Halobacterium salinarum strains NRC-1 and R1, Halorubrum sodomense, Haloarcula valismortis) and of Salinibacter ruber, a red extremely halophilic member of the Bacteria, have been investigated by Raman spectroscopy using 514.5 nm excitation to characterize their carotenoids. The 50-carbon carotenoid α-bacterioruberin was detected as the major carotenoid in all archaeal strains. Raman spectroscopy also detected bacterioruberin as the main pigment in a red pellet of cells collected from a saltern crystallizer pond. Salinibacter contains the C40-carotenoid acyl glycoside salinixanthin (all-E, 2′S)-2′-hydroxy-1′-[6-O-(methyltetradecanoyl) -β-d-glycopyranosyloxy]-3′,4′-didehydro-1′, 2′-dihydro-β,ψ-carotene-4-one), for which the Raman bands assignments of are given here for the first time.

Original languageAmerican English
Pages (from-to)99-103
Number of pages5
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume106
DOIs
StatePublished - Apr 2013

Bibliographical note

Funding Information:
We thank Lily Mana for her assistance in cultivating and harvesting the microorganisms, the Israel Salt Company in Eilat, Israel for allowing access to the salterns, and the staff of the Interuniversity Institute for Marine Sciences of Eilat for logistic support. We thank Adam Culka for technical assistance. This study was supported by a Grant No. P210/10/0467 from the Czech Science Foundation and by institutional support MSM0021620855 from the Ministry of Education of the Czech Republic (to J.J.) .

Keywords

  • Carotenoids
  • Haloarcula
  • Halobacterium
  • Halorubrum
  • Raman spectroscopy
  • Salinibacter

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