Basal splicing factors regulate the stability of mature mRNAs in trypanosomes

Sachin Kumar Gupta; Shai Carmi; Hiba Waldman Ben-Asher; Itai Dov Tkacz; Ilana Naboishchikov; Shulamit Michaeli

doi:10.1074/jbc.M112.416578

Basal splicing factors regulate the stability of mature mRNAs in trypanosomes

Sachin Kumar Gupta
, Shai Carmi
, Hiba Waldman Ben-Asher
, Itai Dov Tkacz
, Ilana Naboishchikov
, Shulamit Michaeli^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Background: Trypanosome trans-splicing depends on basal splicing factors such as U2AF35, U2FA65, and SF1. Results: Transcriptome analyses of RNAi-silenced cells of basal splicing factors reveal differential reliance on factors for trans-splicing and a role for the splicing factors in mRNA stability. Conclusion: Basal splicing factors regulate trans-splicing and mRNA stability. Significance: This is the first study to suggest that basal splicing factors regulate mRNA stability.

Original language	English
Pages (from-to)	4991-5006
Number of pages	16
Journal	Journal of Biological Chemistry
Volume	288
Issue number	7
DOIs	https://doi.org/10.1074/jbc.M112.416578
State	Published - 15 Feb 2013
Externally published	Yes

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title = "Basal splicing factors regulate the stability of mature mRNAs in trypanosomes",

abstract = "Background: Trypanosome trans-splicing depends on basal splicing factors such as U2AF35, U2FA65, and SF1. Results: Transcriptome analyses of RNAi-silenced cells of basal splicing factors reveal differential reliance on factors for trans-splicing and a role for the splicing factors in mRNA stability. Conclusion: Basal splicing factors regulate trans-splicing and mRNA stability. Significance: This is the first study to suggest that basal splicing factors regulate mRNA stability.",

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AU - Gupta, Sachin Kumar

AU - Carmi, Shai

AU - Ben-Asher, Hiba Waldman

AU - Tkacz, Itai Dov

AU - Naboishchikov, Ilana

AU - Michaeli, Shulamit

PY - 2013/2/15

Y1 - 2013/2/15

N2 - Background: Trypanosome trans-splicing depends on basal splicing factors such as U2AF35, U2FA65, and SF1. Results: Transcriptome analyses of RNAi-silenced cells of basal splicing factors reveal differential reliance on factors for trans-splicing and a role for the splicing factors in mRNA stability. Conclusion: Basal splicing factors regulate trans-splicing and mRNA stability. Significance: This is the first study to suggest that basal splicing factors regulate mRNA stability.

AB - Background: Trypanosome trans-splicing depends on basal splicing factors such as U2AF35, U2FA65, and SF1. Results: Transcriptome analyses of RNAi-silenced cells of basal splicing factors reveal differential reliance on factors for trans-splicing and a role for the splicing factors in mRNA stability. Conclusion: Basal splicing factors regulate trans-splicing and mRNA stability. Significance: This is the first study to suggest that basal splicing factors regulate mRNA stability.

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JF - Journal of Biological Chemistry

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Basal splicing factors regulate the stability of mature mRNAs in trypanosomes

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