Beta-adrenergic-stimulated adenylate cyclase activity in normal and EBV-transformed lymphocytes

R. P. Ebstein; M. Steinitz; J. Mintzer; I. Lipshitz; J. Stessman

doi:10.1007/BF01964803

Beta-adrenergic-stimulated adenylate cyclase activity in normal and EBV-transformed lymphocytes

R. P. Ebstein^*, M. Steinitz, J. Mintzer, I. Lipshitz, J. Stessman

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Beta-adrenergic-associated cyclic AMP accumulation was studied in intact lymphocytes before and after transformation with Epstein-Barr virus into immortal cell lines. Although a marked reduction in isoproterenol-stimulated cyclic AMP synthesis was observed in transformed cells, forskolin-stimulated cyclic AMP accumulation was preserved. A parallel loss of¹²⁵-iodocyanopindolol binding sites suggests that the reduction in beta-adrenergic-stimulated AMP synthesis is due to receptor down regulation.

Original language	English
Pages (from-to)	1552-1554
Number of pages	3
Journal	Experientia
Volume	41
Issue number	12
DOIs	https://doi.org/10.1007/BF01964803
State	Published - Dec 1985
Externally published	Yes

Keywords

Epstein-Barr virus
adenylate cyclase
beta-adrenergic receptors
cyclic AMP synthesis
forskolin
lymphocytes

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10.1007/BF01964803

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title = "Beta-adrenergic-stimulated adenylate cyclase activity in normal and EBV-transformed lymphocytes",

abstract = "Beta-adrenergic-associated cyclic AMP accumulation was studied in intact lymphocytes before and after transformation with Epstein-Barr virus into immortal cell lines. Although a marked reduction in isoproterenol-stimulated cyclic AMP synthesis was observed in transformed cells, forskolin-stimulated cyclic AMP accumulation was preserved. A parallel loss of125-iodocyanopindolol binding sites suggests that the reduction in beta-adrenergic-stimulated AMP synthesis is due to receptor down regulation.",

keywords = "Epstein-Barr virus, adenylate cyclase, beta-adrenergic receptors, cyclic AMP synthesis, forskolin, lymphocytes",

author = "Ebstein, {R. P.} and M. Steinitz and J. Mintzer and I. Lipshitz and J. Stessman",

year = "1985",

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AU - Ebstein, R. P.

AU - Steinitz, M.

AU - Mintzer, J.

AU - Lipshitz, I.

AU - Stessman, J.

PY - 1985/12

Y1 - 1985/12

N2 - Beta-adrenergic-associated cyclic AMP accumulation was studied in intact lymphocytes before and after transformation with Epstein-Barr virus into immortal cell lines. Although a marked reduction in isoproterenol-stimulated cyclic AMP synthesis was observed in transformed cells, forskolin-stimulated cyclic AMP accumulation was preserved. A parallel loss of125-iodocyanopindolol binding sites suggests that the reduction in beta-adrenergic-stimulated AMP synthesis is due to receptor down regulation.

AB - Beta-adrenergic-associated cyclic AMP accumulation was studied in intact lymphocytes before and after transformation with Epstein-Barr virus into immortal cell lines. Although a marked reduction in isoproterenol-stimulated cyclic AMP synthesis was observed in transformed cells, forskolin-stimulated cyclic AMP accumulation was preserved. A parallel loss of125-iodocyanopindolol binding sites suggests that the reduction in beta-adrenergic-stimulated AMP synthesis is due to receptor down regulation.

KW - Epstein-Barr virus

KW - adenylate cyclase

KW - beta-adrenergic receptors

KW - cyclic AMP synthesis

KW - forskolin

KW - lymphocytes

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JO - Experientia

JF - Experientia

IS - 12

ER -

Beta-adrenergic-stimulated adenylate cyclase activity in normal and EBV-transformed lymphocytes

Abstract

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