TY - JOUR
T1 - Beta Cells Secrete Significant and Regulated Levels of Insulin for Long Periods when Seeded onto Acellular Micro-Scaffolds
AU - Sionov, Ronit Vogt
AU - Finesilver, Gershon
AU - Sapozhnikov, Lena
AU - Soroker, Avigail
AU - Zlotkin-Rivkin, Efrat
AU - Saad, Yocheved
AU - Kahana, Meygal
AU - Bodaker, Matan
AU - Alpert, Evgenia
AU - Mitrani, Eduardo
N1 - Publisher Copyright:
Copyright 2015, Mary Ann Liebert, Inc.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - The aim of this work is to obtain significant and regulated insulin secretion from human beta cells ex vivo. Long-term culture of human pancreatic islets and attempts at expanding human islet cells normally result in loss of beta-cell phenotype. We propose that to obtain proper ex vivo beta cell function, there is a need to develop three-dimensional structures that mimic the natural islet tissue microenvironment. We here describe the preparation of endocrine micro-pancreata (EMPs) that are made up of acellular organ-derived micro-scaffolds seeded with human intact or enzymatically dissociated islets. We show that EMPs constructed by seeding whole islets, freshly enzymatically-dissociated islets or even dissociated islets grown first in standard monolayer cultures express high levels of key beta-cell specific genes and secrete quantities of insulin per cell similar to freshly isolated human islets in a glucose-regulated manner for more than 3 months in vitro.
AB - The aim of this work is to obtain significant and regulated insulin secretion from human beta cells ex vivo. Long-term culture of human pancreatic islets and attempts at expanding human islet cells normally result in loss of beta-cell phenotype. We propose that to obtain proper ex vivo beta cell function, there is a need to develop three-dimensional structures that mimic the natural islet tissue microenvironment. We here describe the preparation of endocrine micro-pancreata (EMPs) that are made up of acellular organ-derived micro-scaffolds seeded with human intact or enzymatically dissociated islets. We show that EMPs constructed by seeding whole islets, freshly enzymatically-dissociated islets or even dissociated islets grown first in standard monolayer cultures express high levels of key beta-cell specific genes and secrete quantities of insulin per cell similar to freshly isolated human islets in a glucose-regulated manner for more than 3 months in vitro.
UR - http://www.scopus.com/inward/record.url?scp=84946919271&partnerID=8YFLogxK
U2 - 10.1089/ten.tea.2014.0711
DO - 10.1089/ten.tea.2014.0711
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C2 - 26416226
AN - SCOPUS:84946919271
SN - 1937-3341
VL - 21
SP - 2691
EP - 2702
JO - Tissue Engineering - Part A
JF - Tissue Engineering - Part A
IS - 21-22
ER -