Binding sites of human growth hormone and ovine and bovine prolactins in the mammary gland and the liver of lactating dairy cow

A. Gertler*, A. Ashkenazi, Z. Madar

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

Membrane preparations and Triton X-100 solubilized fractions from the mammary gland and liver of the lactating dairy cow were capable of specific binding of [125I]hGH and [125I]oPRL. The specific binding of the latter was significantly lower and could not be increased by higher receptor levels. Displacement studies of [125I]hGh by hGH, bPRL and oPRL revealed that the two latter hormones have a 20-40-fold lower affinity for the receptor than hGH, although strong indications exist that they all bind or the same sites. This feature is unique for cows and does not exist or is much less pronounced in rodents.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume34
Issue number1
DOIs
StatePublished - Jan 1984

Keywords

  • bovine prolactin
  • human growth hormone
  • lactating cow
  • liver
  • mammary gland
  • ovine prolactin
  • receptors

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