TY - JOUR
T1 - Biocatalysis by sol-gel entrapped enzymes
AU - Braun, Sergei
AU - Shtelzer, Sarah
AU - Rappoport, Sara
AU - Avnir, David
AU - Ottolenghi, Michael
PY - 1992
Y1 - 1992
N2 - Attachment of enzymes to insoluble matrix is an essential step in the development of biocatalysts. Transparent xerogels containing various enzymes were obtained by mixing a solution of an enzyme with tetra-methoxy orthosilicate (TMOS) at room temperature followed by gelation and drying. Effective immobilization was usually obtained at initial pH values > 7, where there is a change in the gelation mechanism from predominant hydrolysis/condensation to predominant direct polymerization of silicate precursors. The properties of sol-gel matrix, namely, transparency, large hydrophilic surface and good chemical and thermal stability, make it an ideal material for both biocatalysts and optical sensor devices. An example of a simple optical glucose sensor is demonstrated.
AB - Attachment of enzymes to insoluble matrix is an essential step in the development of biocatalysts. Transparent xerogels containing various enzymes were obtained by mixing a solution of an enzyme with tetra-methoxy orthosilicate (TMOS) at room temperature followed by gelation and drying. Effective immobilization was usually obtained at initial pH values > 7, where there is a change in the gelation mechanism from predominant hydrolysis/condensation to predominant direct polymerization of silicate precursors. The properties of sol-gel matrix, namely, transparency, large hydrophilic surface and good chemical and thermal stability, make it an ideal material for both biocatalysts and optical sensor devices. An example of a simple optical glucose sensor is demonstrated.
UR - http://www.scopus.com/inward/record.url?scp=0026931345&partnerID=8YFLogxK
U2 - 10.1016/S0022-3093(05)80708-2
DO - 10.1016/S0022-3093(05)80708-2
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AN - SCOPUS:0026931345
SN - 0022-3093
VL - 147-148
SP - 739
EP - 743
JO - Journal of Non-Crystalline Solids
JF - Journal of Non-Crystalline Solids
IS - C
ER -