Biocatalytic evolution of a biocatalyst marker: Towards the ultrasensitive detection of immunocomplexes and DNA analysis

Bella Shlyahovsky; Valeri Pavlov; Lubov Kaganovsky; Itamar Willner

doi:10.1002/anie.200600073

Biocatalytic evolution of a biocatalyst marker: Towards the ultrasensitive detection of immunocomplexes and DNA analysis

Bella Shlyahovsky^*, Valeri Pavlov, Lubov Kaganovsky, Itamar Willner

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Sensitive ELISA: A new enzyme-labeled immunosorbant assay (ELISA) protocol has been developed that makes use of two enzymatic reactions to detect antibody-antigen interactions: 1) the conversion of prothrombin to thrombin by ecarin and 2) the hydrolysis of a nonfluorescent substrate 1 to a fluorescent product 2 by thrombin. The system was used to detect bovine serum albumin, telomerase, and DNA with high sensitivity. (Figure Presented).

Original language	English
Pages (from-to)	4815-4819
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	45
Issue number	29
DOIs	https://doi.org/10.1002/anie.200600073
State	Published - 17 Jul 2006

Keywords

Antibodies
Antigens
DNA
Fluorescence
Immunochemistry

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10.1002/anie.200600073

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Biocatalytic evolution of a biocatalyst marker: Towards the ultrasensitive detection of immunocomplexes and DNA analysis

Abstract

Keywords

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