Biocatalytic metal nanopatterning through enzyme-modified microelectrodes

Esteban Malel, Daniel Mandler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


The formation of narrow-size distribution nanomaterials on surfaces in defined patterns is a research area of great interest due to its relevance in many applications such as catalysis, optoelectronics, and sensing devices. Patterning surface with nanostructures has been achieved by numerous techniques including electron-beam lithography, microcontact printing, constructive lithography, and different scanning probe microscopy techniques. Here, we present a different approach by which gold patterns are formed by an enzyme-catalyzed reaction followed by a surface-catalyzed process. Our study takes the advantage of scanning electrochemical microscopy (SECM) where the tip is modified with an enzyme and generates a reductant. The latter participates in an electroless deposition reaction, where AuCl4 is reduced catalyzed by a Pd surface. The result is local deposition of gold patterns made of nanoparticles as soon as the reductant generated by the tip, i.e., hydroquinone, approaches the Pd surface. Two enzymes were used: glucose oxidase (GOx) and alkaline phosphatase (ALP). The entire process was carefully studied and optimized, which enabled a good control of the patterns formed.

Original languageAmerican English
Pages (from-to)2985-2996
Number of pages12
JournalJournal of Solid State Electrochemistry
Issue number11-12
StatePublished - 1 Nov 2020

Bibliographical note

Publisher Copyright:
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.


  • Alkaline phosphatase
  • Biocatalyzed deposition
  • Glucose oxidase
  • Gold nanoparticles
  • Scanning electrochemical microscopy


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