Biochemically active sol-gel glasses: The trapping of enzymes

Sergei Braun; Sara Rappoport; Rivka Zusman; David Avnir; Michael Ottolenghi

doi:10.1016/j.matlet.2007.03.046

Biochemically active sol-gel glasses: The trapping of enzymes

Sergei Braun^*, Sara Rappoport, Rivka Zusman, David Avnir, Michael Ottolenghi

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We describe the preparation and properties of a biochemically active sol-gel glass, obtained by the enzyme alkaline phosphate (ALP) in a polymerizing tetramethoxysilane. The immobilized purified ALP from bovine intestinal mucosa had a 30% activity yield and an improved stability to thermal deactivation compared to a solution. The composite bioactive glass was preserved in water at room temperature for two months without loosing activity. A non-Michaelis-Menten kinetics was observed. The concept of preparing bioactive materials by the sol-gel method seems to be general. Thus, other enzymes (chitinase, asparatase, β-glucose) were successfully trapped.

Original language	English
Pages (from-to)	2843-2846
Number of pages	4
Journal	Materials Letters
Volume	61
Issue number	14-15
DOIs	https://doi.org/10.1016/j.matlet.2007.03.046
State	Published - Jun 2007

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abstract = "We describe the preparation and properties of a biochemically active sol-gel glass, obtained by the enzyme alkaline phosphate (ALP) in a polymerizing tetramethoxysilane. The immobilized purified ALP from bovine intestinal mucosa had a 30% activity yield and an improved stability to thermal deactivation compared to a solution. The composite bioactive glass was preserved in water at room temperature for two months without loosing activity. A non-Michaelis-Menten kinetics was observed. The concept of preparing bioactive materials by the sol-gel method seems to be general. Thus, other enzymes (chitinase, asparatase, β-glucose) were successfully trapped.",

author = "Sergei Braun and Sara Rappoport and Rivka Zusman and David Avnir and Michael Ottolenghi",

year = "2007",

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doi = "10.1016/j.matlet.2007.03.046",

language = "אנגלית",

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journal = "Materials Letters",

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AU - Braun, Sergei

AU - Rappoport, Sara

AU - Zusman, Rivka

AU - Avnir, David

AU - Ottolenghi, Michael

PY - 2007/6

Y1 - 2007/6

N2 - We describe the preparation and properties of a biochemically active sol-gel glass, obtained by the enzyme alkaline phosphate (ALP) in a polymerizing tetramethoxysilane. The immobilized purified ALP from bovine intestinal mucosa had a 30% activity yield and an improved stability to thermal deactivation compared to a solution. The composite bioactive glass was preserved in water at room temperature for two months without loosing activity. A non-Michaelis-Menten kinetics was observed. The concept of preparing bioactive materials by the sol-gel method seems to be general. Thus, other enzymes (chitinase, asparatase, β-glucose) were successfully trapped.

AB - We describe the preparation and properties of a biochemically active sol-gel glass, obtained by the enzyme alkaline phosphate (ALP) in a polymerizing tetramethoxysilane. The immobilized purified ALP from bovine intestinal mucosa had a 30% activity yield and an improved stability to thermal deactivation compared to a solution. The composite bioactive glass was preserved in water at room temperature for two months without loosing activity. A non-Michaelis-Menten kinetics was observed. The concept of preparing bioactive materials by the sol-gel method seems to be general. Thus, other enzymes (chitinase, asparatase, β-glucose) were successfully trapped.

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VL - 61

SP - 2843

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JO - Materials Letters

JF - Materials Letters

IS - 14-15

ER -

Biochemically active sol-gel glasses: The trapping of enzymes

Abstract

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