Biochemically active sol-gel glasses: the trapping of enzymes

Sergei Braun; Sara Rappoport; Rivka Zusman; David Avnir; Michael Ottolenghi

doi:10.1016/0167-577X(90)90002-4

Biochemically active sol-gel glasses: the trapping of enzymes

Sergei Braun^*
, Sara Rappoport
, Rivka Zusman
, David Avnir
, Michael Ottolenghi

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

566 Scopus citations

Abstract

We describe the preparation and properties of a biochemically active sol-gel glass, obtained by trapping the enzyme alkaline phosphatase (ALP) in a polymerizing tetramethoxysilane. The immobilized purified ALP from bovine intestinal mucosa had a 30% activity yield and an improved stability to thermal deactivation compared to a solution. The composite bioactive glass was preserved in water at room temperature for two months without loosing activity. A non-Michaelis-Menten kinetics was observed. The concept of preparing bioactive materials by the sol-gel method seems to be general. Thus, other enzymes (chitinase, aspartase, β-glucosidase) were successfully trapped.

Original language	English
Pages (from-to)	1-5
Number of pages	5
Journal	Materials Letters
Volume	10
Issue number	1-2
DOIs	https://doi.org/10.1016/0167-577X(90)90002-4
State	Published - Sep 1990

Access to Document

10.1016/0167-577X(90)90002-4

Cite this

@article{77cfce2d0a694ddab7aa037bba93dea0,

title = "Biochemically active sol-gel glasses: the trapping of enzymes",

abstract = "We describe the preparation and properties of a biochemically active sol-gel glass, obtained by trapping the enzyme alkaline phosphatase (ALP) in a polymerizing tetramethoxysilane. The immobilized purified ALP from bovine intestinal mucosa had a 30\% activity yield and an improved stability to thermal deactivation compared to a solution. The composite bioactive glass was preserved in water at room temperature for two months without loosing activity. A non-Michaelis-Menten kinetics was observed. The concept of preparing bioactive materials by the sol-gel method seems to be general. Thus, other enzymes (chitinase, aspartase, β-glucosidase) were successfully trapped.",

author = "Sergei Braun and Sara Rappoport and Rivka Zusman and David Avnir and Michael Ottolenghi",

year = "1990",

month = sep,

doi = "10.1016/0167-577X(90)90002-4",

language = "אנגלית",

volume = "10",

pages = "1--5",

journal = "Materials Letters",

issn = "0167-577X",

publisher = "Elsevier B.V.",

number = "1-2",

}

TY - JOUR

T1 - Biochemically active sol-gel glasses

T2 - the trapping of enzymes

AU - Braun, Sergei

AU - Rappoport, Sara

AU - Zusman, Rivka

AU - Avnir, David

AU - Ottolenghi, Michael

PY - 1990/9

Y1 - 1990/9

N2 - We describe the preparation and properties of a biochemically active sol-gel glass, obtained by trapping the enzyme alkaline phosphatase (ALP) in a polymerizing tetramethoxysilane. The immobilized purified ALP from bovine intestinal mucosa had a 30% activity yield and an improved stability to thermal deactivation compared to a solution. The composite bioactive glass was preserved in water at room temperature for two months without loosing activity. A non-Michaelis-Menten kinetics was observed. The concept of preparing bioactive materials by the sol-gel method seems to be general. Thus, other enzymes (chitinase, aspartase, β-glucosidase) were successfully trapped.

AB - We describe the preparation and properties of a biochemically active sol-gel glass, obtained by trapping the enzyme alkaline phosphatase (ALP) in a polymerizing tetramethoxysilane. The immobilized purified ALP from bovine intestinal mucosa had a 30% activity yield and an improved stability to thermal deactivation compared to a solution. The composite bioactive glass was preserved in water at room temperature for two months without loosing activity. A non-Michaelis-Menten kinetics was observed. The concept of preparing bioactive materials by the sol-gel method seems to be general. Thus, other enzymes (chitinase, aspartase, β-glucosidase) were successfully trapped.

UR - https://www.scopus.com/pages/publications/0025493439

U2 - 10.1016/0167-577X(90)90002-4

DO - 10.1016/0167-577X(90)90002-4

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:0025493439

SN - 0167-577X

VL - 10

SP - 1

EP - 5

JO - Materials Letters

JF - Materials Letters

IS - 1-2

ER -

Biochemically active sol-gel glasses: the trapping of enzymes

Abstract

Access to Document

Other files and links

Fingerprint

Cite this