Biocomposite Based on Reduced Graphene Oxide Film Modified with Phenothiazone and Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase for Glucose Sensing and Biofuel Cell Applications

Yehonatan Ravenna; Lin Xia; Jenny Gun; Alexey A. Mikhaylov; Alexander G. Medvedev; Ovadia Lev; Lital Alfonta

doi:10.1021/acs.analchem.5b02949

Biocomposite Based on Reduced Graphene Oxide Film Modified with Phenothiazone and Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase for Glucose Sensing and Biofuel Cell Applications

Yehonatan Ravenna, Lin Xia, Jenny Gun, Alexey A. Mikhaylov, Alexander G. Medvedev, Ovadia Lev^*, Lital Alfonta

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

A novel composite material for the encapsulation of redox enzymes was prepared. Reduced graphene oxide film with adsorbed phenothiazone was used as a highly efficient composite for electron transfer between flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase and electrodes. Measured redox potential for glucose oxidation was lower than 0 V vs Ag/AgCl electrode. The fabricated biosensor showed high sensitivity of 42 mA M^-1 cm^-2, a linear range of glucose detection of 0.5-12 mM, and good reproducibility and stability as well as high selectivity for different interfering compounds. In a semibiofuel cell configuration, the hybrid film generated high power output of 345 μW cm^-2. These results demonstrate a promising potential for this composition in various bioelectronic applications.

Original language	American English
Pages (from-to)	9567-9571
Number of pages	5
Journal	Analytical Chemistry
Volume	87
Issue number	19
DOIs	https://doi.org/10.1021/acs.analchem.5b02949
State	Published - 3 Sep 2015

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© 2015 American Chemical Society.

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10.1021/acs.analchem.5b02949

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Ravenna, Y., Xia, L., Gun, J., Mikhaylov, A. A., Medvedev, A. G., Lev, O., & Alfonta, L. (2015). Biocomposite Based on Reduced Graphene Oxide Film Modified with Phenothiazone and Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase for Glucose Sensing and Biofuel Cell Applications. Analytical Chemistry, 87(19), 9567-9571. https://doi.org/10.1021/acs.analchem.5b02949

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title = "Biocomposite Based on Reduced Graphene Oxide Film Modified with Phenothiazone and Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase for Glucose Sensing and Biofuel Cell Applications",

abstract = "A novel composite material for the encapsulation of redox enzymes was prepared. Reduced graphene oxide film with adsorbed phenothiazone was used as a highly efficient composite for electron transfer between flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase and electrodes. Measured redox potential for glucose oxidation was lower than 0 V vs Ag/AgCl electrode. The fabricated biosensor showed high sensitivity of 42 mA M-1 cm-2, a linear range of glucose detection of 0.5-12 mM, and good reproducibility and stability as well as high selectivity for different interfering compounds. In a semibiofuel cell configuration, the hybrid film generated high power output of 345 μW cm-2. These results demonstrate a promising potential for this composition in various bioelectronic applications.",

author = "Yehonatan Ravenna and Lin Xia and Jenny Gun and Mikhaylov, {Alexey A.} and Medvedev, {Alexander G.} and Ovadia Lev and Lital Alfonta",

note = "Publisher Copyright: {\textcopyright} 2015 American Chemical Society.",

year = "2015",

month = sep,

day = "3",

doi = "10.1021/acs.analchem.5b02949",

language = "American English",

volume = "87",

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Ravenna, Y, Xia, L, Gun, J, Mikhaylov, AA, Medvedev, AG, Lev, O & Alfonta, L 2015, 'Biocomposite Based on Reduced Graphene Oxide Film Modified with Phenothiazone and Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase for Glucose Sensing and Biofuel Cell Applications', Analytical Chemistry, vol. 87, no. 19, pp. 9567-9571. https://doi.org/10.1021/acs.analchem.5b02949

Biocomposite Based on Reduced Graphene Oxide Film Modified with Phenothiazone and Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase for Glucose Sensing and Biofuel Cell Applications. / Ravenna, Yehonatan; Xia, Lin; Gun, Jenny et al.
In: Analytical Chemistry, Vol. 87, No. 19, 03.09.2015, p. 9567-9571.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Biocomposite Based on Reduced Graphene Oxide Film Modified with Phenothiazone and Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase for Glucose Sensing and Biofuel Cell Applications

AU - Ravenna, Yehonatan

AU - Xia, Lin

AU - Gun, Jenny

AU - Mikhaylov, Alexey A.

AU - Medvedev, Alexander G.

AU - Lev, Ovadia

AU - Alfonta, Lital

PY - 2015/9/3

Y1 - 2015/9/3

N2 - A novel composite material for the encapsulation of redox enzymes was prepared. Reduced graphene oxide film with adsorbed phenothiazone was used as a highly efficient composite for electron transfer between flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase and electrodes. Measured redox potential for glucose oxidation was lower than 0 V vs Ag/AgCl electrode. The fabricated biosensor showed high sensitivity of 42 mA M-1 cm-2, a linear range of glucose detection of 0.5-12 mM, and good reproducibility and stability as well as high selectivity for different interfering compounds. In a semibiofuel cell configuration, the hybrid film generated high power output of 345 μW cm-2. These results demonstrate a promising potential for this composition in various bioelectronic applications.

AB - A novel composite material for the encapsulation of redox enzymes was prepared. Reduced graphene oxide film with adsorbed phenothiazone was used as a highly efficient composite for electron transfer between flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase and electrodes. Measured redox potential for glucose oxidation was lower than 0 V vs Ag/AgCl electrode. The fabricated biosensor showed high sensitivity of 42 mA M-1 cm-2, a linear range of glucose detection of 0.5-12 mM, and good reproducibility and stability as well as high selectivity for different interfering compounds. In a semibiofuel cell configuration, the hybrid film generated high power output of 345 μW cm-2. These results demonstrate a promising potential for this composition in various bioelectronic applications.

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DO - 10.1021/acs.analchem.5b02949

M3 - Article

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AN - SCOPUS:84942868049

SN - 0003-2700

VL - 87

SP - 9567

EP - 9571

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 19

ER -

Biocomposite Based on Reduced Graphene Oxide Film Modified with Phenothiazone and Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase for Glucose Sensing and Biofuel Cell Applications

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