Biodegradative mechanism of the brown rot basidiomycete Gloeophyllum trabeum: Evidence for an extracellular hydroquinone-driven fenton reaction

Zohar Kerem; Kenneth A. Jensen; Kenneth E. Hammel

doi:10.1016/S0014-5793(99)00180-5

Biodegradative mechanism of the brown rot basidiomycete Gloeophyllum trabeum: Evidence for an extracellular hydroquinone-driven fenton reaction

Zohar Kerem, Kenneth A. Jensen, Kenneth E. Hammel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

258 Scopus citations

Abstract

We have identified key components of the extracellular oxidative system that the brown rot fungus Gloeophyllum trabeum uses to degrade a recalcitrant polymer, polyethylene glycol, via hydrogen abstraction reactions. G. trabeum produced an extracellular metabolite, 2,5-dimethoxy-1,4-benzoquinone, and reduced it to 2,5-dimethoxyhydroquinone. In the presence of 2,5-dimethoxy-1,4-benzoquinone, the fungus also reduced extracellular Fe³⁺ to Fe²⁺ and produced extracellular H₂O₂. Fe³⁺ reduction and H₂O₂ formation both resulted from a direct, non-enzymatic reaction between 2,5-dimethoxyhydroquinone and Fe³⁺. polyethylene glycol depolymerization by G. trabeum required both 2,5-dimethoxy-1,4-benzoquinone and Fe³⁺ and was completely inhibited by catalase. These results provide evidence that G. trabeum uses a hydroquinone-driven Fenton reaction to cleave polyethylene glycol. We propose that similar reactions account for the ability of G. trabeum to attack lignocellulose. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	American English
Pages (from-to)	49-54
Number of pages	6
Journal	FEBS Letters
Volume	446
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(99)00180-5
State	Published - 5 Mar 1999
Externally published	Yes

Bibliographical note

Funding Information:
We thank D. Dietrich, D. Foster, K. Hirth and D. Litwin for their important technical contributions. We are grateful to A. Paszczynski for sending us data from [20] before publication. The use of trade names herein does not constitute official endorsement of any product by the U.S. Department of Agriculture. This work was supported by the U.S. Department of Energy Grant DE-FG02-94ER20140 (to K.E.H.) and by Postdoctoral Award No. FI-233-96 from the United States-Israel Binational Agricultural Research and Development Fund (to Z.K.).

Keywords

Brown rot
Fenton reaction
Hydroxyl radical
Polyethylene glycol
Quinone redox cycling
Wood decay

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10.1016/S0014-5793(99)00180-5

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title = "Biodegradative mechanism of the brown rot basidiomycete Gloeophyllum trabeum: Evidence for an extracellular hydroquinone-driven fenton reaction",

abstract = "We have identified key components of the extracellular oxidative system that the brown rot fungus Gloeophyllum trabeum uses to degrade a recalcitrant polymer, polyethylene glycol, via hydrogen abstraction reactions. G. trabeum produced an extracellular metabolite, 2,5-dimethoxy-1,4-benzoquinone, and reduced it to 2,5-dimethoxyhydroquinone. In the presence of 2,5-dimethoxy-1,4-benzoquinone, the fungus also reduced extracellular Fe3+ to Fe2+ and produced extracellular H2O2. Fe3+ reduction and H2O2 formation both resulted from a direct, non-enzymatic reaction between 2,5-dimethoxyhydroquinone and Fe3+. polyethylene glycol depolymerization by G. trabeum required both 2,5-dimethoxy-1,4-benzoquinone and Fe3+ and was completely inhibited by catalase. These results provide evidence that G. trabeum uses a hydroquinone-driven Fenton reaction to cleave polyethylene glycol. We propose that similar reactions account for the ability of G. trabeum to attack lignocellulose. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "Brown rot, Fenton reaction, Hydroxyl radical, Polyethylene glycol, Quinone redox cycling, Wood decay",

author = "Zohar Kerem and Jensen, {Kenneth A.} and Hammel, {Kenneth E.}",

note = "Funding Information: We thank D. Dietrich, D. Foster, K. Hirth and D. Litwin for their important technical contributions. We are grateful to A. Paszczynski for sending us data from [20] before publication. The use of trade names herein does not constitute official endorsement of any product by the U.S. Department of Agriculture. This work was supported by the U.S. Department of Energy Grant DE-FG02-94ER20140 (to K.E.H.) and by Postdoctoral Award No. FI-233-96 from the United States-Israel Binational Agricultural Research and Development Fund (to Z.K.). ",

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N1 - Funding Information: We thank D. Dietrich, D. Foster, K. Hirth and D. Litwin for their important technical contributions. We are grateful to A. Paszczynski for sending us data from [20] before publication. The use of trade names herein does not constitute official endorsement of any product by the U.S. Department of Agriculture. This work was supported by the U.S. Department of Energy Grant DE-FG02-94ER20140 (to K.E.H.) and by Postdoctoral Award No. FI-233-96 from the United States-Israel Binational Agricultural Research and Development Fund (to Z.K.).

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N2 - We have identified key components of the extracellular oxidative system that the brown rot fungus Gloeophyllum trabeum uses to degrade a recalcitrant polymer, polyethylene glycol, via hydrogen abstraction reactions. G. trabeum produced an extracellular metabolite, 2,5-dimethoxy-1,4-benzoquinone, and reduced it to 2,5-dimethoxyhydroquinone. In the presence of 2,5-dimethoxy-1,4-benzoquinone, the fungus also reduced extracellular Fe3+ to Fe2+ and produced extracellular H2O2. Fe3+ reduction and H2O2 formation both resulted from a direct, non-enzymatic reaction between 2,5-dimethoxyhydroquinone and Fe3+. polyethylene glycol depolymerization by G. trabeum required both 2,5-dimethoxy-1,4-benzoquinone and Fe3+ and was completely inhibited by catalase. These results provide evidence that G. trabeum uses a hydroquinone-driven Fenton reaction to cleave polyethylene glycol. We propose that similar reactions account for the ability of G. trabeum to attack lignocellulose. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - We have identified key components of the extracellular oxidative system that the brown rot fungus Gloeophyllum trabeum uses to degrade a recalcitrant polymer, polyethylene glycol, via hydrogen abstraction reactions. G. trabeum produced an extracellular metabolite, 2,5-dimethoxy-1,4-benzoquinone, and reduced it to 2,5-dimethoxyhydroquinone. In the presence of 2,5-dimethoxy-1,4-benzoquinone, the fungus also reduced extracellular Fe3+ to Fe2+ and produced extracellular H2O2. Fe3+ reduction and H2O2 formation both resulted from a direct, non-enzymatic reaction between 2,5-dimethoxyhydroquinone and Fe3+. polyethylene glycol depolymerization by G. trabeum required both 2,5-dimethoxy-1,4-benzoquinone and Fe3+ and was completely inhibited by catalase. These results provide evidence that G. trabeum uses a hydroquinone-driven Fenton reaction to cleave polyethylene glycol. We propose that similar reactions account for the ability of G. trabeum to attack lignocellulose. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Brown rot

KW - Fenton reaction

KW - Hydroxyl radical

KW - Polyethylene glycol

KW - Quinone redox cycling

KW - Wood decay

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ER -

Biodegradative mechanism of the brown rot basidiomycete Gloeophyllum trabeum: Evidence for an extracellular hydroquinone-driven fenton reaction

Abstract

Bibliographical note

Keywords

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