Bioluminescent bacterial biosensor for large-scale field deployment

A. J. Agranat; Y. Kabessa; E. Shpigel; B. Shemer; O. Schwartzglass; L. Atamneh; Y. Mizrachi; Y. Uziel; M. Ejzenberg; T. Elad; S. Belkin

doi:10.1117/12.2545954

Bioluminescent bacterial biosensor for large-scale field deployment

A. J. Agranat^*, Y. Kabessa, E. Shpigel, B. Shemer, O. Schwartzglass, L. Atamneh, Y. Mizrachi, Y. Uziel, M. Ejzenberg, T. Elad, S. Belkin

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We describe a biosensing module in which live bacteria, genetically "tailored" to respond to the presence of a specific target material, constitute the core sensing element, reporting their response by bioluminescence. The module is constructed of two channels: an 'induced' channel that measures the bioluminescent light emitted by bacteria exposed to the inspected area, and a 'reference' channel that measures in parallel the bioluminescent light emitted spontaneously by bacteria of the same batch. This enables to overcome signal variations generated by different batches of bacteria, and due to varying environmental operating conditions. A special low-noise optoelectronic circuit was constructed to detect the bioluminescence emitted by the bacteria in both channels. The bacteria are encapsulated in polymer beads that also contain nutrients and water, enabling long-term maintenance-free operation. The beads are packaged in special cassettes at the bottom of the module, so that the induced channel cassette is in direct contact with the ground underneath the module, whereas the reference channel cassette is isolated from the ground. The module contains, in addition, a digital signal processing unit, and a wireless communication unit. The module is designed to operate outdoors as an autonomous network element designed for large scale in-situ deployment. The module described herein was developed for the detection of buried landmines, by sensing the presence of 2,4-dinitrotoluene (DNT) vapors released by the mine, accumulating in the ground above it. Detection of DNT in the sub-ppm range is demonstrated.

Original language	American English
Title of host publication	Frontiers in Biological Detection
Subtitle of host publication	From Nanosensors to Systems XII
Editors	Amos Danielli, Benjamin L. Miller, Sharon M. Weiss
Publisher	SPIE
ISBN (Electronic)	9781510632790
DOIs	https://doi.org/10.1117/12.2545954
State	Published - 2020
Event	Frontiers in Biological Detection: From Nanosensors to Systems XII 2020 - San Francisco, United States Duration: 2 Feb 2020 → 3 Feb 2020

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	11258
ISSN (Print)	1605-7422

Conference

Conference	Frontiers in Biological Detection: From Nanosensors to Systems XII 2020
Country/Territory	United States
City	San Francisco
Period	2/02/20 → 3/02/20

Bibliographical note

Funding Information:
This research was sponsored by the Army Research Office and the Defense Advanced Research Projects Agency (DARPA) Biological Technologies Office (BTO) and was accomplished under Cooperative Agreement Number W911NF-18-2-0002. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of the Army Research Office and the Defense Advanced Research Projects Agency (DARPA) Biological Technologies Office (BTO) or the U.S. Government. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation herein. Work in the Belkin group was also partially supported by the Minerva Center for Bio-Hybrid Complex Systems and by the by NATO Science for Peace and Security Programme project 985042.

Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

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10.1117/12.2545954

Cite this

Agranat, A. J., Kabessa, Y., Shpigel, E., Shemer, B., Schwartzglass, O., Atamneh, L., Mizrachi, Y., Uziel, Y., Ejzenberg, M., Elad, T., & Belkin, S. (2020). Bioluminescent bacterial biosensor for large-scale field deployment. In A. Danielli, B. L. Miller, & S. M. Weiss (Eds.), Frontiers in Biological Detection: From Nanosensors to Systems XII Article 112580I (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11258). SPIE. https://doi.org/10.1117/12.2545954

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title = "Bioluminescent bacterial biosensor for large-scale field deployment",

abstract = "We describe a biosensing module in which live bacteria, genetically {"}tailored{"} to respond to the presence of a specific target material, constitute the core sensing element, reporting their response by bioluminescence. The module is constructed of two channels: an 'induced' channel that measures the bioluminescent light emitted by bacteria exposed to the inspected area, and a 'reference' channel that measures in parallel the bioluminescent light emitted spontaneously by bacteria of the same batch. This enables to overcome signal variations generated by different batches of bacteria, and due to varying environmental operating conditions. A special low-noise optoelectronic circuit was constructed to detect the bioluminescence emitted by the bacteria in both channels. The bacteria are encapsulated in polymer beads that also contain nutrients and water, enabling long-term maintenance-free operation. The beads are packaged in special cassettes at the bottom of the module, so that the induced channel cassette is in direct contact with the ground underneath the module, whereas the reference channel cassette is isolated from the ground. The module contains, in addition, a digital signal processing unit, and a wireless communication unit. The module is designed to operate outdoors as an autonomous network element designed for large scale in-situ deployment. The module described herein was developed for the detection of buried landmines, by sensing the presence of 2,4-dinitrotoluene (DNT) vapors released by the mine, accumulating in the ground above it. Detection of DNT in the sub-ppm range is demonstrated.",

author = "Agranat, {A. J.} and Y. Kabessa and E. Shpigel and B. Shemer and O. Schwartzglass and L. Atamneh and Y. Mizrachi and Y. Uziel and M. Ejzenberg and T. Elad and S. Belkin",

note = "Funding Information: This research was sponsored by the Army Research Office and the Defense Advanced Research Projects Agency (DARPA) Biological Technologies Office (BTO) and was accomplished under Cooperative Agreement Number W911NF-18-2-0002. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of the Army Research Office and the Defense Advanced Research Projects Agency (DARPA) Biological Technologies Office (BTO) or the U.S. Government. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation herein. Work in the Belkin group was also partially supported by the Minerva Center for Bio-Hybrid Complex Systems and by the by NATO Science for Peace and Security Programme project 985042. Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; Frontiers in Biological Detection: From Nanosensors to Systems XII 2020 ; Conference date: 02-02-2020 Through 03-02-2020",

year = "2020",

doi = "10.1117/12.2545954",

language = "American English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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Agranat, AJ, Kabessa, Y, Shpigel, E, Shemer, B, Schwartzglass, O, Atamneh, L, Mizrachi, Y, Uziel, Y, Ejzenberg, M, Elad, T & Belkin, S 2020, Bioluminescent bacterial biosensor for large-scale field deployment. in A Danielli, BL Miller & SM Weiss (eds), Frontiers in Biological Detection: From Nanosensors to Systems XII., 112580I, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11258, SPIE, Frontiers in Biological Detection: From Nanosensors to Systems XII 2020, San Francisco, United States, 2/02/20. https://doi.org/10.1117/12.2545954

Bioluminescent bacterial biosensor for large-scale field deployment. / Agranat, A. J.; Kabessa, Y.; Shpigel, E. et al.
Frontiers in Biological Detection: From Nanosensors to Systems XII. ed. / Amos Danielli; Benjamin L. Miller; Sharon M. Weiss. SPIE, 2020. 112580I (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11258).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Bioluminescent bacterial biosensor for large-scale field deployment

AU - Agranat, A. J.

AU - Kabessa, Y.

AU - Shpigel, E.

AU - Shemer, B.

AU - Schwartzglass, O.

AU - Atamneh, L.

AU - Mizrachi, Y.

AU - Uziel, Y.

AU - Ejzenberg, M.

AU - Elad, T.

AU - Belkin, S.

N1 - Funding Information: This research was sponsored by the Army Research Office and the Defense Advanced Research Projects Agency (DARPA) Biological Technologies Office (BTO) and was accomplished under Cooperative Agreement Number W911NF-18-2-0002. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of the Army Research Office and the Defense Advanced Research Projects Agency (DARPA) Biological Technologies Office (BTO) or the U.S. Government. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation herein. Work in the Belkin group was also partially supported by the Minerva Center for Bio-Hybrid Complex Systems and by the by NATO Science for Peace and Security Programme project 985042. Publisher Copyright: © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

PY - 2020

Y1 - 2020

N2 - We describe a biosensing module in which live bacteria, genetically "tailored" to respond to the presence of a specific target material, constitute the core sensing element, reporting their response by bioluminescence. The module is constructed of two channels: an 'induced' channel that measures the bioluminescent light emitted by bacteria exposed to the inspected area, and a 'reference' channel that measures in parallel the bioluminescent light emitted spontaneously by bacteria of the same batch. This enables to overcome signal variations generated by different batches of bacteria, and due to varying environmental operating conditions. A special low-noise optoelectronic circuit was constructed to detect the bioluminescence emitted by the bacteria in both channels. The bacteria are encapsulated in polymer beads that also contain nutrients and water, enabling long-term maintenance-free operation. The beads are packaged in special cassettes at the bottom of the module, so that the induced channel cassette is in direct contact with the ground underneath the module, whereas the reference channel cassette is isolated from the ground. The module contains, in addition, a digital signal processing unit, and a wireless communication unit. The module is designed to operate outdoors as an autonomous network element designed for large scale in-situ deployment. The module described herein was developed for the detection of buried landmines, by sensing the presence of 2,4-dinitrotoluene (DNT) vapors released by the mine, accumulating in the ground above it. Detection of DNT in the sub-ppm range is demonstrated.

AB - We describe a biosensing module in which live bacteria, genetically "tailored" to respond to the presence of a specific target material, constitute the core sensing element, reporting their response by bioluminescence. The module is constructed of two channels: an 'induced' channel that measures the bioluminescent light emitted by bacteria exposed to the inspected area, and a 'reference' channel that measures in parallel the bioluminescent light emitted spontaneously by bacteria of the same batch. This enables to overcome signal variations generated by different batches of bacteria, and due to varying environmental operating conditions. A special low-noise optoelectronic circuit was constructed to detect the bioluminescence emitted by the bacteria in both channels. The bacteria are encapsulated in polymer beads that also contain nutrients and water, enabling long-term maintenance-free operation. The beads are packaged in special cassettes at the bottom of the module, so that the induced channel cassette is in direct contact with the ground underneath the module, whereas the reference channel cassette is isolated from the ground. The module contains, in addition, a digital signal processing unit, and a wireless communication unit. The module is designed to operate outdoors as an autonomous network element designed for large scale in-situ deployment. The module described herein was developed for the detection of buried landmines, by sensing the presence of 2,4-dinitrotoluene (DNT) vapors released by the mine, accumulating in the ground above it. Detection of DNT in the sub-ppm range is demonstrated.

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U2 - 10.1117/12.2545954

DO - 10.1117/12.2545954

M3 - Conference contribution

AN - SCOPUS:85085263094

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Frontiers in Biological Detection

A2 - Danielli, Amos

A2 - Miller, Benjamin L.

A2 - Weiss, Sharon M.

PB - SPIE

T2 - Frontiers in Biological Detection: From Nanosensors to Systems XII 2020

Y2 - 2 February 2020 through 3 February 2020

ER -

Agranat AJ, Kabessa Y, Shpigel E, Shemer B, Schwartzglass O, Atamneh L et al. Bioluminescent bacterial biosensor for large-scale field deployment. In Danielli A, Miller BL, Weiss SM, editors, Frontiers in Biological Detection: From Nanosensors to Systems XII. SPIE. 2020. 112580I. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2545954

Bioluminescent bacterial biosensor for large-scale field deployment

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