Bioluminescent whole-cell biosensor for on-line water toxicity detection

Ronen Almog; Ramiz Daniel; Sharon Yagur-Kroll; Tal Elad; Sahar Melamed; Shimshon Belkin; Yosi Shacham-Diamand

Bioluminescent whole-cell biosensor for on-line water toxicity detection

Ronen Almog, Ramiz Daniel, Sharon Yagur-Kroll, Tal Elad, Sahar Melamed, Shimshon Belkin, Yosi Shacham-Diamand

Department of Microbiology and Molecular Genetics

Research output: Contribution to conference › Paper › peer-review

1 Scopus citations

Abstract

We present a novel microbial whole-cell sensing system for biodefense and environmental pollution monitoring. Real time water toxicity monitoring is achieved using genetically engineered E. coli bioluminescent cells which emit light when exposed to toxic materials. The illumination intensity and its time dependence are a function of the toxicants dose and type. The bacteria are immobilized in a disposable PDMS biochip that provides live cell maintenance and micro fluidics channels for sample injection. Several bacteria strains have been developed, generating a unique response signature for different toxins.

Original language	English
Pages	597-599
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 12 Oct 2008 → 16 Oct 2008

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	12/10/08 → 16/10/08

Keywords

Bioluminescence
Environmental monitoring
Genetically engineered bacteria
Whole-cell biosensors

Cite this

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abstract = "We present a novel microbial whole-cell sensing system for biodefense and environmental pollution monitoring. Real time water toxicity monitoring is achieved using genetically engineered E. coli bioluminescent cells which emit light when exposed to toxic materials. The illumination intensity and its time dependence are a function of the toxicants dose and type. The bacteria are immobilized in a disposable PDMS biochip that provides live cell maintenance and micro fluidics channels for sample injection. Several bacteria strains have been developed, generating a unique response signature for different toxins.",

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author = "Ronen Almog and Ramiz Daniel and Sharon Yagur-Kroll and Tal Elad and Sahar Melamed and Shimshon Belkin and Yosi Shacham-Diamand",

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AU - Almog, Ronen

AU - Daniel, Ramiz

AU - Yagur-Kroll, Sharon

AU - Elad, Tal

AU - Melamed, Sahar

AU - Belkin, Shimshon

AU - Shacham-Diamand, Yosi

PY - 2008

Y1 - 2008

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AB - We present a novel microbial whole-cell sensing system for biodefense and environmental pollution monitoring. Real time water toxicity monitoring is achieved using genetically engineered E. coli bioluminescent cells which emit light when exposed to toxic materials. The illumination intensity and its time dependence are a function of the toxicants dose and type. The bacteria are immobilized in a disposable PDMS biochip that provides live cell maintenance and micro fluidics channels for sample injection. Several bacteria strains have been developed, generating a unique response signature for different toxins.

KW - Bioluminescence

KW - Environmental monitoring

KW - Genetically engineered bacteria

KW - Whole-cell biosensors

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T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Bioluminescent whole-cell biosensor for on-line water toxicity detection

Abstract

Conference

Keywords

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